2. Academic Validation
  3. Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent

Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent

  • Bioresour Technol. 2019 Sep;288:121548. doi: 10.1016/j.biortech.2019.121548.
Xiaoling Wu 1,  Jun Xiong 1,  Zixuan Huang 1,  Shilin Cao 2,  Minhua Zong 1,  Wenyong Lou 3
Affiliations

Affiliations

  • 1 Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China.
  • 2 School of Food Science and Engineering, Foshan University, Foshan 528000, China.
  • 3 Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, Guangdong, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou 510640, China. Electronic address: wylou@scut.edu.cn.
PMID: 31152956 DOI: 10.1016/j.biortech.2019.121548
Abstract

Deep eutectic solvent (DES), has been considered as a new type of green solvent applied in enzymatic systems. Here, we reported DES-buffer co-solvent as a novel reaction medium for high efficient synthesis of cefaclor by penicilin acylase immobilized on magnetic nanocrystalline cellulose. Effect of DES composition, DES-buffer ratio, temperature, pH, substrate ratio and substrate concentration was systematically investigated. In co-solvent consisting of choline chloride (ChCl):glycol-buffer (7:3, v/v), conversion of 7-ACCA was 94%, synthesis to hydrolysis ratio was 1.8, and yield of cefaclor reached 91%, higher than that in aqueous buffer with optimized yield of 84%, showing the great potential of DES as organic solvent alternative. To the best of our knowledge, this is the first example of biosynthesis of cefaclor in the DES-buffer co-solvent.

Keywords

Biosynthesis; Cefaclor; Deep eutectic solvent; Magnetic nanocrystalline cellulose; Penicilin acylase.

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