2. Academic Validation
  3. Anti-Osteoporotic Effects of Kukoamine B Isolated from Lycii Radicis Cortex Extract on Osteoblast and Osteoclast Cells and Ovariectomized Osteoporosis Model Mice

Anti-Osteoporotic Effects of Kukoamine B Isolated from Lycii Radicis Cortex Extract on Osteoblast and Osteoclast Cells and Ovariectomized Osteoporosis Model Mice

  • Int J Mol Sci. 2019 Jun 6;20(11):2784. doi: 10.3390/ijms20112784.
Eunkuk Park 1 2,  Jeonghyun Kim 3 4,  Mun-Chang Kim 5,  Subin Yeo 6 7,  Jieun Kim 8 9,  Seulbi Park 10 11,  Miran Jo 12 13,  Chun Whan Choi 14,  Hyun-Seok Jin 15,  Sang Woo Lee 16,  Wan Yi Li 17,  Ji-Won Lee 18,  Jin-Hyok Park 19,  Dam Huh 20,  Seon-Yong Jeong 21 22
Affiliations

Affiliations

  • 1 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. jude0815@hotmail.com.
  • 2 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. jude0815@hotmail.com.
  • 3 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. danbi37kjh@hanmail.net.
  • 4 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. danbi37kjh@hanmail.net.
  • 5 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. monotos@hanmail.net.
  • 6 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. snsnans@naver.com.
  • 7 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. snsnans@naver.com.
  • 8 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. eeseul0707@naver.com.
  • 9 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. eeseul0707@naver.com.
  • 10 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. seulbi@ajou.ac.kr.
  • 11 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. seulbi@ajou.ac.kr.
  • 12 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. jjo9313@naver.com.
  • 13 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. jjo9313@naver.com.
  • 14 Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 16229, Korea. cwchoi78@gmail.com.
  • 15 Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea. microchin@hanmail.net.
  • 16 International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea. ethnolee@hanmail.net.
  • 17 Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China. wyli2012@126.com.
  • 18 Korea Food Research Institute, Seongnam 13539, Korea. dnjs0004@naver.com.
  • 19 Dongwoodang Pharmacy Co. Ltd., Yeongchen 38819, Korea. navy9376@hanmail.net.
  • 20 Dongwoodang Pharmacy Co. Ltd., Yeongchen 38819, Korea. herbleader@omniherb.com.
  • 21 Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea. jeongsy@ajou.ac.kr.
  • 22 Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea. jeongsy@ajou.ac.kr.
PMID: 31174394 DOI: 10.3390/ijms20112784
Abstract

Osteoporosis is an abnormal bone remodeling condition characterized by decreased bone density, which leads to high risks of fracture. Previous study has demonstrated that Lycii Radicis Cortex (LRC) extract inhibits bone loss in ovariectomized (OVX) mice by enhancing osteoblast differentiation. A bioactive compound, kukoamine B (KB), was identified from fractionation of an LRC extract as a candidate component responsible for an anti-osteoporotic effect. This study investigated the anti-osteoporotic effects of KB using in vitro and in vivo osteoporosis models. KB treatment significantly increased the osteoblastic differentiation and mineralized nodule formation of osteoblastic MC3T3-E1 cells, while it significantly decreased the osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. The effects of KB on osteoblastic and osteoclastic differentiations under more physiological conditions were also examined. In the co-culture of MC3T3-E1 cells and monocytes, KB promoted osteoblast differentiation but did not affect osteoclast differentiation. In vivo experiments revealed that KB significantly inhibited OVX-induced bone mineral density loss and restored the impaired bone structural properties in osteoporosis model mice. These results suggest that KB may be a potential therapeutic candidate for the treatment of osteoporosis.

Keywords

bone mineral density; herbal medicine; kukoamine B; osteoblast; osteoclast; osteoporosis; ovariectomized mice.

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