2. Academic Validation
  3. Brucella infection regulates peroxiredoxin-5 protein expression to facilitate intracellular survival by reducing the production of nitric oxide and reactive oxygen species

Brucella infection regulates peroxiredoxin-5 protein expression to facilitate intracellular survival by reducing the production of nitric oxide and reactive oxygen species

  • Biochem Biophys Res Commun. 2019 Aug 13;516(1):82-88. doi: 10.1016/j.bbrc.2019.06.026.
Hai Hu 1,  Mingxing Tian 2,  Peng Li 3,  Yanqing Bao 4,  Xiang Guan 5,  Zhengmin Lian 6,  Yi Yin 7,  Chan Ding 8,  Shengqing Yu 9
Affiliations

Affiliations

  • 1 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: wuhusihaixh@163.com.
  • 2 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: tianmx530@126.com.
  • 3 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: 1067276714@qq.com.
  • 4 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: hunterzap@sina.com.
  • 5 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: guanxiang1978@163.com.
  • 6 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: 1048289542@qq.com.
  • 7 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China. Electronic address: yinyisonia@126.com.
  • 8 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, PR China. Electronic address: shoveldeen@shvri.ac.cn.
  • 9 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, PR China. Electronic address: yus@shvri.ac.cn.
PMID: 31196623 DOI: 10.1016/j.bbrc.2019.06.026
Abstract

Peroxiredoxin-5 (Prdx5) is a multifunctional protein involved in oxidative stress, Apoptosis and inflammatory responses. However, how Prdx5 functions during microbial infections is rarely reported. In this study, we demonstrate that Brucella Infection increased Prdx5 expression to promote its intracellular growth in macrophages. Further study show that B. abortus Infection promoted its intracellular growth by decreasing the production of nitric oxide and Reactive Oxygen Species. In addition, the expression of Prdx5 was independent on live Brucella and the type IV secretion system of Brucella. Instead, its expression was regulated by the lipopolysaccharide of Brucella. Moreover, Brucella Infection increased Prdx5 expression in primary macrophage and mice. Collectively, these findings demonstrate for the first time that Prdx5 promotes Brucella intracellular growth by decreasing the production of NO and ROS. This finding provides new insights into the evasive strategies of Brucella and will be useful for the development of novel effective therapeutic approaches to treat Brucella infections.

Keywords

Brucella; Nitric oxide; Prdx5; Reactive oxygen species.

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