2. Academic Validation
  3. Uridine/UMP metabolism and their function on the gut in segregated early weaned piglets

Uridine/UMP metabolism and their function on the gut in segregated early weaned piglets

  • Food Funct. 2019 Jul 17;10(7):4081-4089. doi: 10.1039/c9fo00360f.
Guanya Li 1,  Chunyan Xie 2,  Qinhua Wang 1,  Dan Wan 3,  Yan Zhang 4,  Xin Wu 5,  Yulong Yin 1
Affiliations

Affiliations

  • 1 Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China. w.dan@isa.ac.cn wuxin@isa.ac.cn and Hunan Co-Innovation Center of Safety Animal Production; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
  • 2 Hunan Co-Innovation Center of Safety Animal Production; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
  • 3 Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China. w.dan@isa.ac.cn wuxin@isa.ac.cn.
  • 4 Meiya Haian pharmaceutical Co., Ltd, Hai'an 226600, China.
  • 5 Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China. w.dan@isa.ac.cn wuxin@isa.ac.cn and Hunan Co-Innovation Center of Safety Animal Production; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China and Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330096, China.
PMID: 31231750 DOI: 10.1039/c9fo00360f
Abstract

Uridine monophosphate (UMP) is a major nucleotide analogue in mammalian milk and uridine (UR) is its gastro-intestinal metabolite in vivo. This study aims to investigate the functional effects of UMP and UR on the gut in vitro and in vivo. Twenty-one piglets were randomly allotted into three groups, the control group, UMP group and UR group, and orally administered UMP or UR for 10 days. Results showed that UMP and UR supplements improved the ADG of piglets, and decreased the diarrhea rate. UR increased the jejunum villus length/crypt depth ratio, Claudin-3 and E-cadherin expression, and the pyrimidine nucleotide metabolic Enzymes including CMPK1, RRM2, UPRT, CTPS1 and CTPS2 in the duodenal mucosa. Both the UMP and UR decreased the expression of CAD and RRM2 at the jejunal mucosa. Moreover, UMP and UR increased the Apoptosis ratio of intestinal epithelial cells in in vivo and in vitro experiments. Taken together, oral administration of UR and UMP could improve the small intestinal morphology, promote epithelial cell Apoptosis and renewal of intestinal villus tips, and benefit intestinal development and health thus improving the growth performance and reducing the risk of diarrhea in early-weaned piglets.

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