2. Academic Validation
  3. Anti-inflammatory activity of herb products from Licania rigida Benth

Anti-inflammatory activity of herb products from Licania rigida Benth

  • Complement Ther Med. 2019 Aug:45:254-261. doi: 10.1016/j.ctim.2019.06.001.
Enaide Soares Santos 1,  Cícera Datiane de Morais Oliveira 1,  Irwin Rose Alencar Menezes 1,  Emmily Petícia do Nascimento 1,  Denise Bezerra Correia 1,  Cícero Damon Carvalho de Alencar 1,  Maria de Fátima Sousa 1,  Cícera Norma Fernandes Lima 1,  Álefe Brito Monteiro 2,  Camila Pedroso Estevam de Souza 3,  Gyllyandeson de Araújo Delmondes 1,  Daniel Souza Bezerra 1,  Francisca Adilfa de Oliveira Garcia 3,  Aline Augusti Boligon 4,  José Galberto Martins da Costa 1,  Henrique Douglas Melo Coutinho 5,  Cícero Francisco Bezerra Felipe 2,  Marta Regina Kerntopf 1
Affiliations

Affiliations

  • 1 Regional University of Cariri, Brazil.
  • 2 Federal University of Paraíba, Brazil.
  • 3 The University of western Ontario, Canada.
  • 4 Federal University of Santa Maria, Brazil.
  • 5 Regional University of Cariri, Brazil. Electronic address: hdmcoutinho@gmail.com.
PMID: 31331571 DOI: 10.1016/j.ctim.2019.06.001
Abstract

Purpose: The objective of the present study was to evaluate the systemic anti-inflammatory activity of the hydroalcoholic extract of the leaves of Licania rigida Benth (EHFLR) on models of systemic inflammation in mice.

Methods: The quantitative chemical profiles of phenolic acids and Flavonoids were performed by High-Performance Liquid Chromatography (HPLC). Systemic anti-inflammatory activity was determined from carrageenan and dextran-induced paw edema models and the Animals were orally treated (p.o.) with EHFLR at doses of 25, 50, 100 mg/kg, indomethacin (10 mg/kg) for carrageenan-induced paw edema and promethazine (6 mg/kg) for dextran-induced paw edema. The possible mechanisms involved in the anti-inflammatory action of the extract were evaluated by the paw edema models induced by histamine and arachidonic acid, and by the model of carrageenan-induced peritonitis, where vascular permeability and leukocyte migration to the peritoneal cavity were evaluated.

Results: The results of the HPLC identified the presence of phenolic acids and Flavonoids, with chlorogenic acid (1.16%) and Caempferol (0.81%) as the main constituents. From the results, it was concluded that the extract has an LD50 ≥5000 mg/kg when administered orally in mice as this dose did not trigger deaths in any of the observed groups. EHFLR (25 mg/kg) showed a significant antiderematogenic effect on histamine and arachidonic acid-induced paw edema at the third hour of the tests, with a percentage of inhibition of 46.64% and 18.33%, respectively. The extract (25 mg/kg, p.o.) also significantly reduced vascular permeability and leukocyte migration in the peritoneal cavity.

Conclusions: It is concluded that EHFLR exerts a systemic anti-inflammatory action, which seems to depend, at least in part, on the inhibition of arachidonic acid metabolism and the action of vasoactive amines. In addition, the extract reduced the leukocyte migration in the peritoneal cavity, indicating that its action may be linked to the inhibition of pro-inflammatory cytokines.

Keywords

Flavonoids; Inflammation; Licania rigida; Phenolic acids.

Figures
Products