2. Academic Validation
  3. The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

The Sirt1/P53 Axis in Diabetic Intervertebral Disc Degeneration Pathogenesis and Therapeutics

  • Oxid Med Cell Longev. 2019 Sep 9;2019:7959573. doi: 10.1155/2019/7959573.
Zengjie Zhang 1 2 3,  Jialiang Lin 1 2 3,  Majid Nisar 1 2 3,  Tingting Chen 2 4,  Tianzhen Xu 2 5,  Gang Zheng 1 2 3,  Chenggui Wang 1 2 3,  Haiming Jin 1 2 3,  Jiaoxiang Chen 1 2 3,  Weiyang Gao 1 2 3,  Naifeng Tian 1 2 3,  Xiangyang Wang 1 2 3,  Xiaolei Zhang 1 2 3 6
Affiliations

Affiliations

  • 1 Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, 325027 Zhejiang Province, China.
  • 2 Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, Zhejiang Province, China.
  • 3 The Second School of Medicine, Wenzhou Medical University, China.
  • 4 The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang Street, Ouhai District, Wenzhou, 325027 Zhejiang Province, China.
  • 5 The Third Affiliated Hospital and Ruian People's Hospital of Wenzhou Medical University, Wansong Road 108#, Ruian, Zhejiang Province, China.
  • 6 Chinese Orthopaedic Regenerative Medicine Society, Hangzhou, China.
PMID: 31583043 DOI: 10.1155/2019/7959573
Abstract

Intervertebral disc degeneration (IDD) is one of the major causes of low back pain. Diabetes is a risk factor for IDD and may aggravate IDD in rats; however, the mechanism is poorly understood. Previously, we demonstrated that Apoptosis and senescence were increased in diabetic nucleus pulposus (NP) tissues; in the current study, we found that hyperglycaemia may promote the incidence of Apoptosis and senescence in NP cells in vitro. Meanwhile, the acetylation of P53, a master transcription factor of Apoptosis and senescence, was also found increased in diabetic NP tissues in vivo as well as in hyperglycaemic NP cells in vitro. SIRT1 is an NAD+-dependent deacetylase, and we showed that the expression of SIRT1 was decreased in NP tissues, while hyperglycaemia could suppress the expression and activity of SIRT1 in NP cells. Furthermore, we demonstrated that butein may inhibit acetylation of P53 and protect NP cells against hyperglycaemia-induced Apoptosis and senescence through SIRT1 activation, as the SIRT1 Inhibitor Ex527 may counteract the protective effect of butein in hyperglycaemic NP cells. An in vivo study showed that butein could ameliorate the IDD process in diabetic rats, while SIRT1 was increased and acetyl-p53 was decreased in NP tissues in butein-treated rats. These results indicate that the SIRT1/P53 axis is involved in the pathogenesis of diabetic IDD and may serve as a therapeutic target for diabetic IDD.

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