1. Academic Validation
  2. Thiohydantoin and Hydantoin Derivatives from the Roots of Armoracia rusticana and Their Neurotrophic and Anti-neuroinflammatory Activities

Thiohydantoin and Hydantoin Derivatives from the Roots of Armoracia rusticana and Their Neurotrophic and Anti-neuroinflammatory Activities

  • J Nat Prod. 2019 Nov 22;82(11):3020-3024. doi: 10.1021/acs.jnatprod.9b00527.
Tae Hyun Lee 1 Zahra Khan 2 3 Sun Yeou Kim 2 3 Kang Ro Lee 1
Affiliations

Affiliations

  • 1 Natural Products Laboratory, School of Pharmacy , Sungkyunkwan University , Suwon 16419 , Republic of Korea.
  • 2 Gachon Institute of Pharmaceutical Science , Gachon University , Incheon 21936 , Republic of Korea.
  • 3 College of Pharmacy , Gachon University , #191, Hambakmoero , Yeonsu-gu, Incheon 21936 , Republic of Korea.
Abstract

Two new thiohydantoins (1 and 3) and three new hydantoins (2, 4, and 5) along with three known compounds (6-8) were isolated from roots of horseradish. Physical data analysis including NMR (1H and 13C NMR, 1H-1H COSY, HSQC, and HMBC), HRESIMS, and ECD were employed for structure elucidation of the new compounds 1-5. Potential neuroprotective effects of all compounds (1-8) on nerve growth factor (NGF) induction in C6 glioma were also evaluated. Among these compounds, 1b and 2a exhibited potent NGF secretion stimulation activities (NGF secretion levels: 153.59 ± 5.44% and 141.99 ± 5.21%, respectively). Their anti-neuroinflammatory activities were also assessed based on their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-stimulated murine microglia. Compound 7 marginally inhibited NO production with an IC50 value of 32.6 μM.

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