Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo

Background: Antiviral drugs such as rupintrivir may have an immune-modulatory effect in experimentally induced allergic asthma with subsequent RV Infection. We infected lung slices of house-dust Mite (HDM)-sensitized asthmatic mice ex vivo with human rhinovirus (RV) and investigated the effect of the Antiviral drug rupintrivir on RV-induced cytokine response in lung tissue of HDM-sensitized mice ex vivo.

Methods: Mice were sensitized with HDM. Precision-cut lung slices (PCLS) were prepared from HDM-sensitized or non-sensitized mice. Lung slices were infected ex vivo with RV or RV together with rupintrivir. Modulation of immune responses was evaluated by cytokine secretion 48 h post Infection.

Results: In vivo HDM sensitization resulted in a T_H-2/T_H-17-dominated cytokine response that persisted in PCLS ex vivo. RV Infection of PCLS from non-sensitized mice resulted in the induction of an Antiviral and pro-inflammatory immune response, as indicated by the secretion of IFN-α, IFN-β, IFN-γ, TNF-α, MCP-1, IP-10, IL-10, and IL-17A. In contrast, PCLS from HDM-sensitized mice showed an attenuated Antiviral response, but exaggerated IL-4, IL-6, and IL-10 secretion upon Infection. Rupintrivir inhibited exaggerated pro-inflammatory cytokine IL-6 and T_H-2 cytokine IL-4 in HDM-sensitized mice.

Conclusions: In summary, this study demonstrates that treatment with rupintrivir influences virus-induced IL-4 and IL-6 cytokine release under experimental conditions ex vivo.