2. Academic Validation
  3. Ammonia regulates chicken tracheal cell necroptosis via the LncRNA-107053293/MiR-148a-3p/FAF1 axis

Ammonia regulates chicken tracheal cell necroptosis via the LncRNA-107053293/MiR-148a-3p/FAF1 axis

  • J Hazard Mater. 2020 Mar 15;386:121626. doi: 10.1016/j.jhazmat.2019.121626.
Wei Wang 1,  Qunxiang Shi 1,  Shengchen Wang 1,  Hongfu Zhang 2,  Shiwen Xu 3
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
  • 2 State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China. Electronic address: zhanghongfu@caas.cn.
  • 3 College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address: shiwenxu@neau.edu.cn.
PMID: 31791863 DOI: 10.1016/j.jhazmat.2019.121626
Abstract

Ammonia (NH3) is a known harmful gas that causes injury to the respiratory system. Ammonia also exists in haze, forming secondary organic aerosols. However, the specific damage caused by NH3 in chicken trachea has not been determined. The regulatory mechanism of ceRNA and its multiple roles have been proposed in many pathomechanisms; therefore, we investigated the functional role of ceRNA in chicken trachea after NH3 inhalation. Broiler chicken trachea exposed to NH3 was selected as the research object. The pathological ultrastructure was observed by transmission electron microscopy. Transcriptome analyses were applied and referenced, and lncRNA-107053293 and miR-148a-3p and FAF1 were selected. A dual-luciferase reporter assay verified the target relationship. Real-time quantitative PCR (RT-PCR) and western blotting were performed to examine the expression levels of Necroptosis genes, such as RIPK1, RIPK3, MLKL, Caspase 8, and FADD. Our results indicated that lncRNA-107053293 regulated Necroptosis by acting as a competing endogenous RNA of miR-148a-3p. FAF1, as a gene target of miR-148a-3p, also affects Necroptosis.

Keywords

CeRNA; NH(3); Necroptosis; Non-coding RNA transcriptome.

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