2. Academic Validation
  3. Regulation of Il-10 gene expression by Il-6 via Stat3 in grass carp head kidney leucocytes

Regulation of Il-10 gene expression by Il-6 via Stat3 in grass carp head kidney leucocytes

  • Gene. 2020 May 30;741:144579. doi: 10.1016/j.gene.2020.144579.
Chao Wen 1,  Ning Gan 1,  Tingting Zeng 1,  Mengyuan Lv 1,  Na Zhang 1,  Hong Zhou 1,  Anying Zhang 1,  Xinyan Wang 2
Affiliations

Affiliations

  • 1 Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
  • 2 Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China. Electronic address: wangxyhku@uestc.edu.cn.
PMID: 32171822 DOI: 10.1016/j.gene.2020.144579
Abstract

Interleukin (IL)-10 is a critical anti-inflammatory and late cytokine being produced after the proinflammatory mediators while IL-6 is a promptly synthesized cytokine in response to inflammation in mammals. This chronological expression of interleukin (Il)-6 and IL-10 was also found in grass carp head kidney leucocytes (HKLs) treated by heat-killed Aeromonas hydrophila, supporting the possible interplay between grass carp (gc)IL-6 and gcIl-10 in HKLs. Our further findings were in agreement with this hypothesis that recombinant gcIl-6 (rgcIl-6) promptly and transiently increased gcil10 mRNA levels in grass carp HKLs. Moreover, rgcIl-6 enhanced its own mRNA level and this self-enhancement of gcil6 mRNA level could be partially blocked by rgcIl-10. These results collectively suggest that gcIl-10 production stimulated by gcIl-6 may provide a negative feedback to gcIl-6 production. Interestingly, rgcIl-6 significantly decreased gcil10 mRNA levels in grass carp HKLs after the treatment for 12 and 24 h in contrast to its enhancement of gcil10 levels after the treatment for 3 h. Involvement of STAT3 but not MEK, p38 MAPK or JNK pathway in the increase of gcil10 mRNA levels by rgcIl-6 was revealed by using the signaling pathway inhibitors. This was supported by the fact that rgcIl-6 stimulated STAT3 phosphorylation in grass carp HKLs. Furthermore, rgcIl-6 had no effect on the stability of gcil10 mRNA after the treatment for 3 to 36 h while it increased gcil10 promoter activity after the treatment for 24 h. Taken these data together, gcIl-6 can stimulate IL-10 production at early stage but subsequently inhibit il10 mRNA expression in grass carp HKLs, shedding LIGHT on the dynamic regulation of il10 mRNA expression by IL-6 in fish immune cells.

Keywords

Gene expression; Grass carp; Head kidney leucocytes; Il-10; Il-6; Signal transduction.

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