2. Academic Validation
  3. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

  • Inorg Chem. 2020 Apr 6;59(7):4527-4535. doi: 10.1021/acs.inorgchem.9b03679.
Maria V Babak 1,  Pauline Le Faouder 1,  Xavier Trivelli 2,  Gopalakrishnan Venkatesan 3,  Stanislav I Bezzubov 4,  Mohammed Kajjout 1,  Artem L Gushchin 5 6,  Muhammad Hanif 7,  Olivier Poizat 8,  Hervé Vezin 8,  Christian Rolando 1
Affiliations

Affiliations

  • 1 Univ. Lille, CNRS, USR 3290, MSAP, Miniaturisation pour la Synthèse l'Analyse et la Protèomique, F-59 000 Lille, France.
  • 2 Univ. Lille, CNRS, INRA, Centrale Lille, ENSCL, Univ. Artois, FR 2638 - IMEC - Institut Michel-Eugène Chevreul, F-59000 Lille, France.
  • 3 Department of Pharmacy, National University of Singapore, Lower Kent Ridge Road, 18 Science Drive 2, Singapore 119260.
  • 4 Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninskii pr. 31, Moscow 119991, Russia.
  • 5 Nikolaev Institute of Inorganic Chemistry, Siberian Branch of Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia.
  • 6 Novosibirsk State University, 2 Pirogov Street, 630090 Novosibirsk, Russia.
  • 7 School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
  • 8 Univ. Lille, CNRS, UMR 8516 - LASIRe - Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement, F-59000 Lille, France.
PMID: 32181663 DOI: 10.1021/acs.inorgchem.9b03679
Abstract

The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

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