Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds

A novel atmospheric pressure ionization mass spectrometry based on wire desorption and electrospray ionization (WD-ESI) for direct analysis was developed to characterize chemical compounds with different polarities and thermal stabilities at atmospheric pressure. This technique is a variant of the thermal desorption electrospray ion source developed by Shiea et al. One large improvement is that the heating speed (>500 °C/s) of the thermal desorption in this work is extremely fast, using a self-heating metal wire, with which sample solution can splash from the surface to form small droplets and thus the analytes can be protected from thermal decomposition. With this feature, we have successfully achieved soft ionization of highly polar organic and biological compounds such as aflatoxin, small Peptides, and even large proteins from complex matrices. The simple structure and self-cleaning capability of the WD-ESI source make it ideal for on-site screening in various applications such as food safety and biodrug testing, especially when coupled with a transportable mass spectrometer.