Neurotensin

Name: Neurotensin
Brand: MedChemExpress
SKU: HY-P0234
Rating: 5.0 (2 reviews)

Cat. No.: HY-P0234 Purity: 98.68%: FAQs
Data Sheet SDS COA Handling Instructions

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Neurotensin, a gut tridecapeptide, acts as a potent cellular mitogen for various colorectal and pancreatic cancers which possess high-affinity neurotensin receptors (NTR).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Neurotensin Chemical Structure

CAS No. : 39379-15-2

Size	Price	Stock	Quantity
1 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 277	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 554	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Neurotensin

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NTR1 NTR2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Neurotensin, a gut tridecapeptide, acts as a potent cellular mitogen for various colorectal and pancreatic cancers which possess high-affinity neurotensin receptors (NTR).

IC₅₀ & Target

Neurotensin receptors (NTR)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	4.9 x 10^-10 M Compound: Neurotensin	Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method	[PMID: 27876250]
CHO-K1	EC₅₀	> 100 nM Compound: NT	Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay	[PMID: 25499438]
CHO-K1	EC₅₀	0.04 nM Compound: NT	Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay	[PMID: 24856674]
CHO-K1	EC₅₀	0.23 nM Compound: Neurotensin, NT	Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay	[PMID: 19195889]
CHO-K1	EC₅₀	0.31 μM Compound: neurotensin, NT	Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay	[PMID: 18849166]
CHO-K1	IC₅₀	114 nM Compound: NT	Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay	[PMID: 25157640]
CHO-K1	IC₅₀	18.5 nM Compound: NT	Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay	[PMID: 24856674]
CHO-K1	IC₅₀	42 nM Compound: NT	Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay	[PMID: 25499438]
CHO-K1	IC₅₀	6.03 nM Compound: neurotensin, NT	Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay	[PMID: 18849166]
HT-29	EC₅₀	1.98 nM Compound: NT	Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay	[PMID: 19290594]

In Vitro

Neurotensin induces the expression of MIP-2, MCP-1, IL-1β and TNFα in murine microglial cells and stimulates IL-8 secretion in a non-transformed colon epithelial cell line stably transfected with the NTR. The high-affinity NTR, a member of the G-protein coupled receptor (GPCR) family, is present in a majority of human pancreatic and colorectal cancers, suggesting that Neurotensin (NT) may act in an endocrine fashion to affect tumor growth. Acting through the NTR1, Neurotensin is known to stimulate various signal transduction pathways, including intracellular calcium ([Ca²⁺]_i), the mitogen-activated protein kinases (MAPKs), ERK and JNK, and various PKC isoforms. Treatment of HCT116 cells with Neurotensin (100 nM) significantly increases HCT116 cell migration (~3-fold) compared with vehicle treatment; pretreatment with Curcumin (10 μM) blocksthe stimulatory effect of NT on HCT116 cell migration. ctivation of MEK/ERK by NT and downstream induction of AP-1 transcription factors contributes to the proliferative effects of Neurotensin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

1672.92

Formula

C₇₈H₁₂₁N₂₁O₂₀

CAS No.

39379-15-2

Appearance

Solid

Color

White to off-white

Sequence

{Pyr}-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu

Sequence Shortening

{Pyr}-LYENKPRRPYIL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (19.92 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.5978 mL	2.9888 mL	5.9776 mL
5 mM	0.1196 mL	0.5978 mL	1.1955 mL
10 mM	0.0598 mL	0.2989 mL	0.5978 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 20 mg/mL (11.96 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.68%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (255 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Wang X, et al. Curcumin inhibits neurotensin-mediated interleukin-8 production and migration of HCT116 human colon cancer cells. Clin Cancer Res. 2006 Sep 15;12(18):5346-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.5978 mL	2.9888 mL	5.9776 mL	14.9439 mL
	5 mM	0.1196 mL	0.5978 mL	1.1955 mL	2.9888 mL
	10 mM	0.0598 mL	0.2989 mL	0.5978 mL	1.4944 mL
	15 mM	0.0399 mL	0.1993 mL	0.3985 mL	0.9963 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Neurotensin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neurotensin39379-15-2Neurotensin ReceptorInhibitorinhibitorinhibit

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Neurotensin

Customer Review

Neurotensin Related Antibodies

2 Publications Citing Use of MCE Neurotensin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Neurotensin Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Neurotensin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Neurotensin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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