2. Academic Validation
  3. Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

  • In Vivo. 2020 Jul-Aug;34(4):1823-1833. doi: 10.21873/invivo.11977.
Yi-Xi Gong  # 1,  Yue Liu  # 1,  Ying-Hua Jin  # 2,  Mei-Hua Jin 1,  Ying-Hao Han 1,  Jing Li 1,  Gui-Nan Shen 1,  Dan-Ping Xie 1,  Chen-Xi Ren 1,  Li-Yun Yu 1,  Dong-Seok Lee 3,  Ji-Su Kim 4,  Yu-Jin Jo 4,  Jeongwoo Kwon 5,  Jaihyung Lee 6,  Yang Ho Park 7,  Taeho Kwon 8,  Yu-Dong Cui 9,  Hu-Nan Sun 9
Affiliations

Affiliations

  • 1 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing, P.R. China.
  • 2 Library and Information Center, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
  • 3 School of Life Sciences, KNU Creative BioResearch Group (BK21 plus project), Kyungpook National University, Daegu, Republic of Korea.
  • 4 Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, Republic of Korea.
  • 5 Department of Animal Sciences, Chungbuk Natonal University, Cheongju, Republic of Korea.
  • 6 Haeam Convalescence Hospital, Gyeonggi, Republic of Korea.
  • 7 Park Yang Ho BRM Institute, Seoul, Republic of Korea.
  • 8 Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, Republic of Korea kwon@kribb.re.kr cuiyudong@126.com sunhunan76@163.com.
  • 9 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing, P.R. China kwon@kribb.re.kr cuiyudong@126.com sunhunan76@163.com.
  • # Contributed equally.
PMID: 32606152 DOI: 10.21873/invivo.11977
Abstract

Background/aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P. quassioides ethanol extract on SiHa cervical Cancer cell Apoptosis.

Materials and methods: The P. quassioides extract-induced Apoptosis was analyzed using the MTT assay, fluorescence microscopy, flow cytometry and western blotting.

Results: P. quassioides extract induced cellular Apoptosis by increasing the accumulation of cellular and mitochondrial Reactive Oxygen Species (ROS) levels and inhibiting ATP synthesis. Pretreatment with N-Acetylcysteine (NAC), a classic antioxidant, decreased the intracellular ROS production and inhibited Apoptosis. In addition, the p38 MAPK signaling pathway is a key in the Apoptosis of SiHa cells induced by the P. quassioides extract.

Conclusion: The P. quassioides extract exerts its anti-cancer properties on SiHa cells through ROS-mitochondria axis and p38 MAPK signaling. Our data provide a new insight for P. quassioides as a therapeutic strategy for cervical Cancer treatment.

Keywords

Picrasma quassioides; ROS; apoptosis; cervical cancer; mitochondria.

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