2. Academic Validation
  3. Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

  • Carbohydr Polym. 2020 Dec 1;249:116874. doi: 10.1016/j.carbpol.2020.116874.
Xiong Gao 1,  Jiayi Qi 2,  Chi-Tang Ho 3,  Bin Li 2,  Jingjing Mu 4,  Yuting Zhang 2,  Huiping Hu 4,  Weipeng Mo 4,  Zhongzheng Chen 5,  Yizhen Xie 6
Affiliations

Affiliations

  • 1 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China; Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, 510663, China.
  • 2 College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, 510642, China.
  • 3 Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ, 08901, USA.
  • 4 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.
  • 5 College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, 510642, China. Electronic address: zhongzhengch@scau.edu.cn.
  • 6 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China; Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, 510663, China. Electronic address: xieyizhen@126.com.
PMID: 32933694 DOI: 10.1016/j.carbpol.2020.116874
Abstract

Ganoderma leucocontextum is a new species of Ganoderma discovered in 2014. Up to now, the structural characteristics and immunoregulatory activity of its Polysaccharides remain virtually unknown. In this study, a water-soluble polysaccharide termed, GLP-3, was purified from G. leucocontextum by ultrafiltration and column chromatography. The results revealed that GLP-3 mainly consisted of glucose (92.7 %) and its weight average molecular weight was 159.7 kDa. The structural analysis indicated that the backbone of GLP-3 was →4)-α-D-Glcp-(1→4,6)-β-D-Glcp-(1→ with a β-Glcp-(1→ branch. Atomic force microscopy and Congo red experiments revealed that GLP-3 might possess a globular structure with triple-helix conformation in water. Moreover, GLP-3 was recognized by Toll-like Receptor 2 (TLR2) and exerted immunomodulatory effects via activating mitogen-activated protein kinases (MAPKs), phosphatidylinositol-3-kinase (PI3K)/Akt and nuclear factor-κB (NF-κB) signaling pathways in RAW 264.7 macrophages. Collectively, these results suggested that GLP-3 could be developed as a potential functional food ingredient for immunomodulation.

Keywords

Ganoderma leucocontextum; Immunostimulatory activity; Polysaccharide; Structure; Toll-like receptor 2.

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