1. Academic Validation
  2. Bovine Stearoyl-CoA Desaturase 1 Promotes Adipogenesis by Activating the PPARγ Receptor

Bovine Stearoyl-CoA Desaturase 1 Promotes Adipogenesis by Activating the PPARγ Receptor

  • J Agric Food Chem. 2020 Oct 28;68(43):12058-12066. doi: 10.1021/acs.jafc.0c05147.
Yu Zhang 1 Yan Zheng 1 Xiaoyu Wang 1 Ju Qiu 1 Chengcheng Liang 1 Gong Cheng 1 2 Hongbao Wang 1 2 Chunping Zhao 1 2 Wucai Yang 1 2 Linsen Zan 1 2 Anning Li 1 2
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
  • 2 National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
Abstract

Stearoyl-CoA desaturase 1 (SCD1) is a rate-limiting Enzyme that mainly catalyzes the saturated fatty acids (SFAs) into the monounsaturated fatty acids (MUFAs). The expression level of SCD1 is positively correlated with the marbling score. However, the functional mechanism of SCD1 in adipogenesis is still unclear. In this study, we identified SCD1 as highly expressed in subcutaneous and visceral fat, peaking at 2 days after differentiation in bovine stromal vascular fraction (SVF) cells. When the SCD1 was overexpressed in bovine SVF cells, lipid droplets accumulation was increased from 142.46 ± 21.77 to 254.89 ± 11.75 μg/mg (P < 0.01). Further, the expression levels of FABP4, FASN, and ACCα were increased (P < 0.01), while the expression of PPARγ or C/EBPα was not changed at mRNA or protein level (P > 0.05). Dual-luciferase reporter assay showed that the activity of the PPARγ receptor was enhanced by 3.69 times (P < 0.01). Moreover, the contents of palmitoleate (C16:1) and oleate (C18:1) were significantly increased (P < 0.05). Furthermore, 100 μM exogenous oleate increased the lipid accumulation by 22.28 times (P < 0.01). These results suggest that oleate is probably a strong ligand of the PPARγ receptor to enhance adipogenesis.

Keywords

PPARγ; SCD1; adipogenesis; cattle; oleate.

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