2. Academic Validation
  3. ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2

ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2

  • FEBS Lett. 2021 Jan;595(2):169-182. doi: 10.1002/1873-3468.13970.
Wen Zhang 1 2,  Shou-Song Tao 1 2,  Ting Wang 3,  Jie Zhang 1 2,  Xian Liu 2,  Ya-Ting Li 1 2,  Hui Chen 2,  Yi-Qun Zhan 2,  Miao Yu 2,  Chang-Hui Ge 4,  Chang-Yan Li 2,  Guang-Ming Ren 2,  Xiao-Ming Yang 1 2 3,  Rong-Hua Yin 2
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, China.
  • 2 State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, China.
  • 3 School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
  • 4 Beijing Institute of Radiation Medicine, China.
PMID: 33107021 DOI: 10.1002/1873-3468.13970
Abstract

BRCA1/BRCA2-containing complex subunit 3 (BRCC3) is a lysine 63-specific Deubiquitinase involved in multiple biological processes, such as DNA repair and immune responses. However, the regulation mechanism for BRCC3 protein stability is still unknown. Here, we demonstrate that BRCC3 is mainly degraded through the ubiquitin-proteasome pathway. The HECT-type E3 ubiquitin Ligase WWP2 modulates BRCC3 ubiquitination and degradation. ABRO1, a subunit of the BRCC36 isopeptidase complex (BRISC), competes with WWP2 to bind to BRCC3, thereby preventing WWP2-mediated BRCC3 ubiquitination and enhancing BRCC3 stability. Functionally, we show that lentivirus-mediated overexpression of WWP2 in murine macrophages inhibits NLRP3 inflammasome activation by decreasing BRCC3 protein level. This study provides the first insights into the regulation of BRCC3 stability and expands our knowledge about the physiological function of WWP2.

Keywords

ABRO1; BRCC3; NLRP3 inflammasome; WWP2; stability; ubiquitination.

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