1. Academic Validation
  2. Dihydrotanshinone I Is Effective against Drug-Resistant Helicobacter pylori In Vitro and In Vivo

Dihydrotanshinone I Is Effective against Drug-Resistant Helicobacter pylori In Vitro and In Vivo

  • Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01921-20. doi: 10.1128/AAC.01921-20.
Peipei Luo 1 2 3 4 5 Yanqiang Huang 1 2 3 5 6 Xudong Hang 1 2 3 5 Qian Tong 1 2 3 5 Liping Zeng 1 2 3 5 Jia Jia 1 2 3 5 Guoxin Zhang 4 5 Hongkai Bi 7 2 3 5
Affiliations

Affiliations

  • 1 Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 2 Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 3 Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 4 Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • 5 Helicobacter pylori Research Center, Nanjing Medical University, Nanjing, Jiangsu, China.
  • 6 Research Center for the Prevention and Treatment of Drug Resistant Microbial Infection, Youjiang Medical University for Nationalities, Baise, Guangxi, China.
  • 7 Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China hkbi@njmu.edu.cn.
Abstract

Helicobacter pylori is a major global pathogen and has been implicated in gastritis, peptic ulcer, and gastric carcinoma. The efficacy of the extensive therapy of H. pylori Infection with Antibiotics is compromised by the development of drug resistance and toxicity toward human gut microbiota, which urgently demands novel and selective Antibacterial strategies. The present study was mainly performed to assess the in vitro and in vivo effects of a natural herbal compound, dihydrotanshinone I (DHT), against standard and clinical H. pylori strains. DHT demonstrated effective Antibacterial activity against H. pyloriin vitro (MIC50/90, 0.25/0.5 μg/ml), with no development of resistance during continuous serial passaging. Time-kill curves showed strong time-dependent bactericidal activity for DHT. Also, DHT eliminated preformed biofilms and killed biofilm-encased H. pylori cells more efficiently than the conventional Antibiotic metronidazole. In mouse models of multidrug-resistant H. pylori Infection, dual therapy with DHT and omeprazole showed in vivo killing efficacy superior to that of the standard triple-therapy approach. Moreover, DHT treatment induces negligible toxicity against normal tissues and exhibits a relatively good safety index. These results suggest that DHT could be suitable for use as an anti-H. pylori agent in combination with a Proton Pump Inhibitor to eradicate multidrug-resistant H. pylori.

Keywords

Helicobacter pylori; Salvia miltiorrhiza; dihydrotanshinone I; drug resistance.

Figures
Products