2. Academic Validation
  3. Dehydroepiandrosterone attenuates LPS-induced inflammatory responses via activation of Nrf2 in RAW264.7 macrophages

Dehydroepiandrosterone attenuates LPS-induced inflammatory responses via activation of Nrf2 in RAW264.7 macrophages

  • Mol Immunol. 2021 Mar;131:97-111. doi: 10.1016/j.molimm.2020.12.023.
Ji Cao 1,  Qian Li 1,  Xuehuai Shen 2,  Yao Yao 1,  Longlong Li 1,  Haitian Ma 3
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
  • 2 Institute of Animal Husbandry and Veterinary Science, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Anhui Academy of Agricultural Sciences, Hefei, 230001, China.
  • 3 Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: mahaitian@njau.edu.cn.
PMID: 33461765 DOI: 10.1016/j.molimm.2020.12.023
Abstract

Dehydroepiandrosterone (DHEA) is the major steroid hormone in humans and Animals, which can regulate the body's inflammatory responses. However, the detail mechanism of this beneficial function is still poorly understood. The present study aimed to explore the anti-inflammation effect of DHEA and its underlying molecular mechanism in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The findings showed that DHEA significantly inhibited the inflammation-related mediators production and pro-inflammatory cytokines expression level. Further research found that DHEA obviously blocked the LPS-stimulated PI3K/Akt, MAPK and NF-κB activation in RAW 264.7 cells. Meanwhile, DHEA enhanced the autophagy-dependent Keap1 protein degradation, subsequently activated the Nrf2 pathway to alleviate the redox imbalance and inflammatory responses. In conclusion, our data demonstrated that DHEA suppresses inflammatory responses through the activation of Nrf2 and inhibition of NF-κB in LPS-stimulated macrophages.

Keywords

Dehydroepiandrosterone; Inflammation; Lipopolysaccharide; NF-κB; Nrf2.

Figures
Products