2. Academic Validation
  3. A novel methymycin analog, 12-ketomethymycin N-oxide, produced by the heterologous expression of the large pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900

A novel methymycin analog, 12-ketomethymycin N-oxide, produced by the heterologous expression of the large pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900

  • Biosci Biotechnol Biochem. 2021 Mar 24;85(4):890-894. doi: 10.1093/bbb/zbaa111.
Reiko Ueoka 1,  Junko Hashimoto 2,  Ikuko Kozone 2,  Takuya Hashimoto 1,  Kei Kudo 1,  Noritaka Kagaya 1,  Hikaru Suenaga 1,  Haruo Ikeda 3,  Kazuo Shin-Ya 1 4
Affiliations

Affiliations

  • 1 National Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
  • 2 Japan Biological Informatics Consortium (JBIC), Tokyo, Japan.
  • 3 Kitasato Institute for Life Sciences, Kitasato University, Kanagawa, Japan.
  • 4 Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
PMID: 33590846 DOI: 10.1093/bbb/zbaa111
Abstract

A novel methymycin analog, 12-ketomethymycin N-oxide, was produced by the heterologous expression of the pikromycin/methymycin biosynthetic gene cluster of Streptomyces sp. AM4900 together with 12-ketomethymycin, which was only isolated by the biotransformation of the synthetic intermediate before. Their structures were determined by the spectroscopic data and the chemical derivatization. 12-Ketomethymycin showed a weak cytotoxicity against SKOV-3 and Jurkat cells, although its N-oxide analog did not show any activity. Both showed no Antibacterial activities against Escherichia coli and Micrococcus luteus.

Keywords

actinomycete; heterologous expression; isolation; structure elucidation.

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