2. Academic Validation
  3. Activity In Vitro of 2-Chloro-N-[4-(4-Chlorophenyl)-2-Thiazolyl]Acetamide Against Promastigotes of Leishmania mexicana: An Apoptosis Inducer

Activity In Vitro of 2-Chloro-N-[4-(4-Chlorophenyl)-2-Thiazolyl]Acetamide Against Promastigotes of Leishmania mexicana: An Apoptosis Inducer

  • Acta Parasitol. 2021 Sep;66(3):1068-1073. doi: 10.1007/s11686-020-00328-6.
Mario Daniel Caba-Flores 1,  Delia Hernández-Romero 2,  Aracely López-Monteon 3,  Esmeralda Sánchez-Pavón 2,  Diana Carolina Valdez-Ortega 2,  Jaime López-Domínguez 3,  Víctor Adolfo Romero-Cruz 3,  Alberto Yair Limón-Flores 4,  Ángel Trigos 1,  Angel Ramos-Ligonio 5
Affiliations

Affiliations

  • 1 Centro de Investigaciones Biomédicas, Dr. Luis Castelazo Ayala, Industrial Las Ánimas, Universidad Veracruzana, C.P. 91190, Xalapa, Veracruz, Mexico.
  • 2 LADISER de Química Orgánica y Biotecnología, Facultad de Ciencias Químicas, Universidad Veracruzana, Prolongación Oriente 6, No. 1009, Col. Rafael Alvarado, CP 94340, Orizaba, Veracruz, Mexico.
  • 3 LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Prolongación Oriente 6, No. 1009, Col. Rafael Alvarado, CP 94340, Orizaba, Veracruz, Mexico.
  • 4 Facultad de Medicina y Hospital Universitario, Servicio de Inmunología, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
  • 5 LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Prolongación Oriente 6, No. 1009, Col. Rafael Alvarado, CP 94340, Orizaba, Veracruz, Mexico. angramos@uv.mx.
PMID: 33616814 DOI: 10.1007/s11686-020-00328-6
Abstract

Purpose: Leishmaniasis is an infectious disease transmitted by insects that proliferate mainly in impoverished environments of tropical climates. In the absence of an effective vaccine, pharmacological treatment is the main tool to combat this disease. The objective of this work was to analyze the anti-leishmanial activity of 2-chloro-N-[4-(4-chlorophenyl)-2-thiazolyl] acetamide (AT) in promastigotes of Leishmania mexicana.

Methods: The biological activity of the compound was evaluated using a sulphorhodamine B cytotoxicity test and the integrity of the erythrocytes was evaluated by a lysis test. The anti-trypanosomatid activity was evaluated in vitro, a cell death assay was performed by flow cytometry (IP/Annexin V stain) and a Parasite growth recovery assay was performed.

Results: The AT showed a CC50 value of 0.031 µM for HeLa cells after 24 h of exposure, which did not induce erythrocyte lysis. On the other hand, the AT showed an IC50 value of 0.086 µM for L. mexicana (promastigote form) after 24 h of interaction. The compound was capable of inducing Apoptosis in the parasites and did not allow recovery after 24 h of exposure.

Conclusion: This study provides valuable information with the objective of developing new drugs for the treatment of this disease, although more research on this molecule is needed to improve its biological activity.

Keywords

Aminothiazole derivatives; Apoptosis; Infectious disease; Leishmaniasis; Neglected tropical diseases.

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