2. Academic Validation
  3. Decreased expression of ARID1A invasively downregulates the expression of ribosomal proteins in hepatocellular carcinoma

Decreased expression of ARID1A invasively downregulates the expression of ribosomal proteins in hepatocellular carcinoma

  • Biomark Med. 2021 May;15(7):497-508. doi: 10.2217/bmm-2020-0464.
Jing Zhao 1,  Weiran Xu 2,  Yu Zhang 3,  Xiaomin Lv 4,  Yiran Chen 5,  Gaoda Ju 5,  Fang Yang 6,  Li Lin 2,  Xiaosong Rao 7,  Ziwei Guo 2,  Tao Xing 5,  Li Li 2,  Jun Liang 2
Affiliations

Affiliations

  • 1 Department of Medical Oncology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266000, China.
  • 2 Department of Oncology, Peking University International Hospital, Peking University, Beijing, 102206, China.
  • 3 Department of Medical Oncology & Radiation Sickness, Peking University Third Hospital, Peking University, Beijing, 100191, China.
  • 4 Department of Neurology, The First Hospital of Jilin University, Jilin University, Changchun, 130021, China.
  • 5 Department of Medical Oncology, Key Laboratory of Carcinogenesis & Translational Research (Ministry of Education/Beijing), Beijing, 102206, China.
  • 6 Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences, Suzhou, 215000, China.
  • 7 Department of Pathology, Peking University International Hospital, Peking University, Beijing, 102206, China.
PMID: 33769075 DOI: 10.2217/bmm-2020-0464
Abstract

Background: There was increasing evidence showing that ARID1A alterations correlated with higher tumor mutational burden, but there were limited studies focusing on the adaptive mechanisms for tumor cells to survive under excessive genomic alterations. Materials & methods: To further explore the adaptive mechanisms under ARID1A alterations, we performed RNA Sequencing in ARID1A knockdown hepatocellular carcinoma cell lines, and demonstrated that decreased expression of ARID1A controlled global ribosomal proteins synthesis. The results were further confirmed by quantitative reverse transcription-PCR and bioinformatic analysis in The Cancer Genome Atlas Liver Hepatocellular Carcinoma database. Conclusion: The present study was the first to demonstrate that ARID1A might be involved in the translation pathway and served as an adaptive mechanism for tumor cells to survive under stress.

Keywords

ARID1A; HCC; ribosomal proteins; translation.

Figures
Products