2. Academic Validation
  3. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis

RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis

  • Cell Death Dis. 2021 Apr 1;12(4):336. doi: 10.1038/s41419-021-03642-7.
Qian Liang  # 1,  Yun Wang  # 1,  Yingsi Lu 1,  Qingqing Zhu 1,  Wenlin Xie 2,  Nannan Tang 3,  Lifen Huang 3,  Tailai An 4,  Di Zhang 1,  Anqi Yan 5,  Shaoyu Liu 6,  Liping Ye 7,  Chengming Zhu 8
Affiliations

Affiliations

  • 1 Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
  • 2 Department of Pathology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
  • 3 Division of Hematology/Oncology, Department of Pediatrics, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
  • 4 Center of Digestive Diseases, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
  • 5 Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
  • 6 Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.
  • 7 Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. yelp5@mail.sysu.edu.cn.
  • 8 Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. zhuchm3@mail.sysu.edu.cn.
  • # Contributed equally.
PMID: 33795653 DOI: 10.1038/s41419-021-03642-7
Abstract

The tumor necrosis factor (TNF) receptor superfamily member 11a (TNFRSF11a, also known as RANK) was demonstrated to play an important role in tumor metastasis. However, the specific function of RANK in colorectal Cancer (CRC) metastasis and the underlying mechanism are unknown. In this study, we found that RANK expression was markedly upregulated in CRC tissues compared with that in matched noncancerous tissues. Increased RANK expression correlated positively with metastasis, higher TNM stage, and worse prognosis in patients with CRC. Overexpression of RANK promoted CRC cell metastasis in vitro and in vivo, while knockdown of RANK decreased cell migration and invasion. Mechanistically, RANK overexpression significantly upregulated the expression of tartrate-resistant Acid Phosphatase 5 (TRAP/ACP5) in CRC cells. Silencing of ACP5 in RANK-overexpressing CRC cells attenuated RANK-induced migration and invasion, whereas overexpression of ACP5 increased the migration and invasion of RANK-silencing cells. The ACP5 expression was transcriptionally regulated by Calcineurin/nuclear factor of activated T cells c1 (NFATC1) axis. The inhibition of Calcineurin/NFATC1 significantly decreased ACP5 expression, and attenuated RANK-induced cell migration and invasion. Furthermore, RANK induced Phospholipase C-gamma (PLCγ)-mediated inositol-1,4,5-trisphosphate receptor (IP3R) axis and stromal interaction molecule 1 (STIM1) to evoke calcium (CA2+) oscillation. The RANK-mediated intracellular CA2+ mobilization stimulated Calcineurin to dephosphorylate NFATC1 and induce NFATC1 nuclear translocation. Both blockage of PLCγ-IP3R axis and STIM1 rescued RANK-induced NFATC1 nuclear translocation, ACP5 expression, and cell metastasis. Our study revealed the functional expression of RANK in human CRC cells and demonstrated that RANK induced the CA2+-calcineurin/NFATC1-ACP5 axis in the regulation of CRC metastasis, that might be amenable to therapeutic targeting.

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