2. Membrane Transporter/Ion Channel Neuronal Signaling TGF-beta/Smad Epigenetics Anti-infection Apoptosis
  3. Calcium Channel PKC Bacterial Apoptosis Antibiotic
  4. Ionomycin

Ionomycin  (Synonyms: SQ23377)

Cat. No.: HY-13434 Purity: ≥99.0%
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Ionomycin (SQ23377) is a potent, selective calcium ionophore and an antibiotic produced by Streptomyces conglobatus. Ionomycin (SQ23377) is highly specific for divalent cations (Ca>Mg>Sr=Ba). Ionomycin (SQ23377) promotes apoptosis. Ionomycin also induces the activation of protein kinase C (PKC).

For research use only. We do not sell to patients.

Ionomycin Chemical Structure

Ionomycin Chemical Structure

CAS No. : 56092-81-0

Size Price Stock Quantity
Solvent
1mg (14.1 mM * 100 μL in Ethanol) USD 50 In-stock
Solvent
5mg (14.1 mM * 500 μL in Ethanol) USD 200 In-stock
Solvent
10mg (14.1 mM * 1 mL in Ethanol) USD 380 In-stock
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Based on 64 publication(s) in Google Scholar

Other Forms of Ionomycin:

Ionomycin Related Antibodies

Top Publications Citing Use of Products

63 Publications Citing Use of MCE Ionomycin

IF

    Ionomycin purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2022 Dec 8;303:116031.  [Abstract]

    Calcium imaging in HEK293T cells expressing E649A, E652K, and E652D mutants responds to 10 nM capsaicin, the cocktail of 10 nM capsaicin and 30 μM s-RhTx, 10 μM capsaicin, and 10 μM ionomycin, respectively.

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    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

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    Description

    Ionomycin (SQ23377) is a potent, selective calcium ionophore and an antibiotic produced by Streptomyces conglobatus. Ionomycin (SQ23377) is highly specific for divalent cations (Ca>Mg>Sr=Ba). Ionomycin (SQ23377) promotes apoptosis. Ionomycin also induces the activation of protein kinase C (PKC)[1][2][3].

    IC50 & Target

    Calcium ionophore[1]
    In Vitro

    Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus[1].
    Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment[2].
    Ionomycin-mediated cleavage and exosome release. Following Ionomycin exposure, medium conditioned by SKOV3ip cells had increased amounts of exosomes containing the L1-32 cleavage fragment[4].
    Ionomycin also phosphorylate p38 MAPK by Ca2+ influx through SOCE, leading to suppression of TNF-α-induced NF-κB phosphorylation[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ionomycin Related Antibodies
    Clinical Trial
    Molecular Weight

    709.01

    Formula

    C41H72O9
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    O=C(O)CC[C@@H](C)C[C@H](C)C[C@H](C)C(/C=C(O)/[C@H](C)C[C@H](C)C/C=C/[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@]([C@@H]2O[C@](C)([C@H](O)C)CC2)(C)O1)=O
    Structure Classification
    Initial Source

    Streptomyces conglobatus
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Solvent & Solubility
    In Vitro: 

    Ethanol : 100 mg/mL (141.04 mM; Need ultrasonic)

    DMSO : 100 mg/mL (141.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: ≥99.0%

    SDS (393 KB)

    COA (242 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 1.4104 mL 7.0521 mL 14.1042 mL 35.2604 mL
    5 mM 0.2821 mL 1.4104 mL 2.8208 mL 7.0521 mL
    10 mM 0.1410 mL 0.7052 mL 1.4104 mL 3.5260 mL
    15 mM 0.0940 mL 0.4701 mL 0.9403 mL 2.3507 mL
    20 mM 0.0705 mL 0.3526 mL 0.7052 mL 1.7630 mL
    25 mM 0.0564 mL 0.2821 mL 0.5642 mL 1.4104 mL
    30 mM 0.0470 mL 0.2351 mL 0.4701 mL 1.1753 mL
    40 mM 0.0353 mL 0.1763 mL 0.3526 mL 0.8815 mL
    50 mM 0.0282 mL 0.1410 mL 0.2821 mL 0.7052 mL
    60 mM 0.0235 mL 0.1175 mL 0.2351 mL 0.5877 mL
    80 mM 0.0176 mL 0.0882 mL 0.1763 mL 0.4408 mL
    100 mM 0.0141 mL 0.0705 mL 0.1410 mL 0.3526 mL
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    Ionomycin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ionomycin56092-81-0SQ23377SQ 23377SQ-23377Calcium ChannelPKCBacterialApoptosisAntibioticCa2+ channelsCa channelsProtein kinase CcleavageexosomecalciumionophoreantibioticInhibitorinhibitorinhibit

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