Role of microRNA-25 in high glucose cultured Müller glia

Aim: To investigate the role of microRNA-25 (miR-25) in proliferation and Apoptosis of retinal Müller glia (MG) under high glucose condition.

Methods: The purity of the cultured cells was verified by immunocytochemistry and flow cytometry using Antibodies that specifically recognized MG. The expression level of miR-25 under normal and high glucose conditions were validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR). miR-25 mimics and negative control were transfected into MG and multiple functional experiments including cell counting kit-8 assay, EDU assay, and flow cytometry were conducted to explore the effects of miR-25 on the proliferation and Apoptosis of high glucose cultured MG (HGMG).

Results: Immunocytochemistry and flow cytometry confirmed the high purity of primary cultured MG. RT-PCR results showed that the expression level of miR-25 was significantly repressed in HGMG, while over-expression of miR-25 by miR-25 mimic markedly inhibited the high glucose induced cell Apoptosis and promoted the proliferation of MG.

Conclusion: The expression level of miR-25 is significantly downregulated in HGMG and its overexpression could attenuate the high glucose damages on MG by promoting proliferation and reducing Apoptosis.