2. Academic Validation
  3. Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2

Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2

  • Cells. 2021 May 24;10(6):1303. doi: 10.3390/cells10061303.
Rizwan Ali 1,  Hajar Al Zahrani 1,  Tlili Barhoumi 1,  Alshaimaa Alhallaj 1,  Abdullah Mashhour 1,  Musaad A Alshammari 2,  Yasser A Alshawakir 3,  Omar Baz 1,  Abdullah H Alanazi 4,  Abdul Latif Khan 4,  Hassan Al Nikhli 4,  Mohammed A Al Balwi 4 5 6,  Lolwah Al Riyees 7,  Mohamed Boudjelal 1
Affiliations

Affiliations

  • 1 King Abdullah International Medical Research Center (KAIMRC), Medical Research Core Facility and Platforms (MRCFP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11481, Saudi Arabia.
  • 2 Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
  • 3 Experimental Surgery and Animal Lab, College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia.
  • 4 Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), MNGHA, Riyadh 11426, Saudi Arabia.
  • 5 KAIMRC, Department of Medical Genomic Research, KSAU-HS, Riyadh 11481, Saudi Arabia.
  • 6 College of Medicine, KSAU-HS, Riyadh 11481, Saudi Arabia.
  • 7 Department of Surgery, KAMC, MNGHA, Riyadh 11426, Saudi Arabia.
PMID: 34073849 DOI: 10.3390/cells10061303
Abstract

In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized Cancer cell lines are the best and easiest model for studying Cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast Cancer cell line, KAIMRC2. This cell line is derived from a Saudi Arabian female breast Cancer patient with invasive ductal carcinoma. Immunocytochemistry showed a significant ratio of the KAIMRC2 cells' expressing key breast epithelial and Cancer Stem Cells (CSCs) markers, including CD47, CD133, CD49f, CD44, and ALDH-1A1. Gene and protein expression analysis showed overexpression of ABC transporter and AKT-PI3Kinase as well as JAK/STAT signaling pathways. In contrast, the absence of the tumor suppressor genes p53 and p73 may explain their high proliferative index. The mice model also confirmed the tumorigenic potential of the KAIMRC2 cell line, and drug tolerance studies revealed few very potent candidates. Our results confirmed an aggressive phenotype with metastatic potential and Cancer stem cell-like characteristics of the KAIMR2 cell line. Furthermore, we have also presented potent small molecule inhibitors, especially Ryuvidine, that can be further developed, alone or in synergy with Other potent inhibitors, to target multiple cancer-related pathways.

Keywords

KAIMRC2; breast cancer; cell line; characterization; drug treatment; metastatic; stem cells; triple negative.

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