Ryuvidine

Cat. No.: HY-100624 Purity: 99.40%: FAQs
Data Sheet SDS COA Handling Instructions

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Ryuvidine is a potent inhibitor of SET domain-containing protein 8(SETD8) with an IC₅₀ of 0.5 µM and suppresses monomethylation of H₄K₂₀ in vitro. Ryuvidine also inhibits CDK4 with an IC₅₀ of 6.0 μM and is cytotoxic against a range of human cancer cells.

For research use only. We do not sell to patients.

Ryuvidine Chemical Structure

CAS No. : 265312-55-8

Size	Price	Stock	Quantity
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
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10 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

Biological Activity
Purity & Documentation
References
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Description

Ryuvidine is a potent inhibitor of SET domain-containing protein 8(SETD8) with an IC₅₀ of 0.5 µM and suppresses monomethylation of H₄K₂₀ in vitro^[1]. Ryuvidine also inhibits CDK4 with an IC₅₀ of 6.0 μM and is cytotoxic against a range of human cancer cells^[2].

IC₅₀ & Target

CDK4

6.0 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	0.37 μM Compound: Ryuvidine	Inhibition of SETD8 (unknown origin) using [3H]SAM and HeLa nucleosomes as substrates incubated for 1 hr Inhibition of SETD8 (unknown origin) using [3H]SAM and HeLa nucleosomes as substrates incubated for 1 hr	[PMID: 35525212]
HepG2	IC₅₀	1.2 μg/mL Compound: 4d	In vitro cytotoxicity against HepG2 cells (Hepatocarcinoma from ATCC) by MTT assay In vitro cytotoxicity against HepG2 cells (Hepatocarcinoma from ATCC) by MTT assay	[PMID: 10915058]
Sf9	IC₅₀	> 20 μM Compound: Ryuvidine	Inhibition of His-tagged CDK1/cyclin B1 (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of His-tagged CDK1/cyclin B1 (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]
Sf9	IC₅₀	> 20 μM Compound: Ryuvidine	Inhibition of His-tagged CDK2/cyclin E (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of His-tagged CDK2/cyclin E (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]
Sf9	IC₅₀	> 20 μM Compound: Ryuvidine	Inhibition of GST-tagged CDK5/p25 (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of GST-tagged CDK5/p25 (unknown origin) expressed in Baculovirus infected Sf9 cells using histone H1 as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]
Sf9	IC₅₀	> 20 μM Compound: Ryuvidine	Inhibition of GST-tagged CDK7/cyclinH/MAT1 (unknown origin) expressed in Baculovirus infected Sf9 cells using YSPTSPS-2 KK peptide as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of GST-tagged CDK7/cyclinH/MAT1 (unknown origin) expressed in Baculovirus infected Sf9 cells using YSPTSPS-2 KK peptide as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]
Sf9	IC₅₀	> 20 μM Compound: Ryuvidine	Inhibition of GST-tagged CDK9/CyclinT1 (unknown origin) expressed in Baculovirus infected Sf9 cells using YSPTSPS-2 KK peptide as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of GST-tagged CDK9/CyclinT1 (unknown origin) expressed in Baculovirus infected Sf9 cells using YSPTSPS-2 KK peptide as substrate as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]
Sf9	IC₅₀	9.5 μM Compound: Ryuvidine	Inhibition of GST-tagged CDK4/cyclin D1 (unknown origin) expressed in Baculovirus infected Sf9 cells using RPPTLSPIPHIPR peptide as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay Inhibition of GST-tagged CDK4/cyclin D1 (unknown origin) expressed in Baculovirus infected Sf9 cells using RPPTLSPIPHIPR peptide as substrate in presence of [gamma-33P]-ATP by radiometric filter binding assay	[PMID: 30234987]

Molecular Weight

284.33

Formula

C₁₅H₁₂N₂O₂S

CAS No.

265312-55-8

Appearance

Solid

Color

Brown to black

SMILES

O=C1C=C(C(C2=C1SC(C)=N2)=O)NC3=CC=C(C)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (43.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5170 mL	17.5852 mL	35.1704 mL
5 mM	0.7034 mL	3.5170 mL	7.0341 mL
10 mM	0.3517 mL	1.7585 mL	3.5170 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (247 KB) HNMR (243 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Ryu CK, et al. 5-Arylamino-2-methyl-4,7-dioxobenzothiazoles as inhibitors of cyclin-dependent kinase 4 and cytotoxic agents. Bioorg Med Chem Lett. 2000 Mar 6;10(5):461-4. [Content Brief]

[2]. Blum G, et al. Small-molecule inhibitors of SETD8 with cellular activity. ACS Chem Biol. 2014 Nov 21;9(11):2471-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5170 mL	17.5852 mL	35.1704 mL	87.9260 mL
	5 mM	0.7034 mL	3.5170 mL	7.0341 mL	17.5852 mL
	10 mM	0.3517 mL	1.7585 mL	3.5170 mL	8.7926 mL
	15 mM	0.2345 mL	1.1723 mL	2.3447 mL	5.8617 mL
	20 mM	0.1759 mL	0.8793 mL	1.7585 mL	4.3963 mL
	25 mM	0.1407 mL	0.7034 mL	1.4068 mL	3.5170 mL
	30 mM	0.1172 mL	0.5862 mL	1.1723 mL	2.9309 mL
	40 mM	0.0879 mL	0.4396 mL	0.8793 mL	2.1982 mL

Ryuvidine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ryuvidine265312-55-8CDKCyclin dependent kinaseInhibitorinhibitorinhibit

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Ryuvidine

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Ryuvidine Related Antibodies

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References

Customer Review

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Complete Stock Solution Preparation Table

Ryuvidine Related Classifications

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Customer Review

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