2. Academic Validation
  3. Effect of resveratrol on the biofilm formation and physiological properties of avian pathogenic Escherichia coli

Effect of resveratrol on the biofilm formation and physiological properties of avian pathogenic Escherichia coli

  • J Proteomics. 2021 Oct 30;249:104357. doi: 10.1016/j.jprot.2021.104357.
Xiangchun Ruan 1,  Xiaoling Deng 2,  Meiling Tan 2,  Youwei Wang 2,  Jidong Hu 2,  Ying Sun 2,  Chengbo Yu 2,  Meishi Zhang 2,  Nuohao Jiang 2,  Runshen Jiang 3
Affiliations

Affiliations

  • 1 Laboratory of Veterinary Pharmacology and Toxicology, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei, Anhui 230036, China. Electronic address: rxc@ahau.edu.cn.
  • 2 Laboratory of Veterinary Pharmacology and Toxicology, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • 3 Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: jiangrunshen@ahau.edu.cn.
PMID: 34450330 DOI: 10.1016/j.jprot.2021.104357
Abstract

Avian pathogenic Escherichia coli (APEC) is widely distributed, causing great economic losses to the poultry industry. The formation of APEC biofilms causes chronic, persistent, and repeated infections in the clinic, making treatment difficult. Resveratrol is a natural product, which has good health benefits including antimicrobial, anti-inflammatory, and cardiovascular activities. Resveratrol shows efficient inhibition of Bacterial biofilm formation. However, a comprehensive understanding of the proteomic properties of APEC treated resveratrol is still lacking. In this study, APEC cells treated by resveratrol were investigated using a label-free differential proteomic method. Several proteins, including those related to a two-component system and chemotaxis, were found to be implicated in APEC biofilm formation. In addition, the physiological properties were significantly changed in terms of purine, pyruvate, and glyoxylate and dicarboxylate metabolism in APEC. Data are available via ProteomeXchange with the identifier PXD025706. We speculated that pyruvate dehydrogenase might be a potential target to inhibit Escherichia coli biofilm formation. Overall, our results indicated that resveratrol inhibits APEC biofilm formation by regulating the levels of proteins in two-component systems, especially chemotaxis proteins. The results showed that resveratrol had a potential application in inhibiting the biofilm formation of APEC. SIGNIFICANCE: This study elucidated the mechanism of resveratrol inhibiting biofilm formation of avian pathogenic Escherichia coli (APEC) based on a label-free differential proteomics. It was indicated that resveratrol inhibits APEC biofilm formation by regulating the levels of proteins in two component systems, especially chemotaxis proteins. Meanwhile, we speculated that pyruvate dehydrogenase might be a potential target to inhibit Escherichia coli biofilm formation. It shows that resveratrol has a potential application prospect in inhibiting the biofilm formation of APEC.

Keywords

Avian pathogenic Escherichia coli; Biofilm; Label-free analysis; Proteomics; Resveratrol.

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