Effect of Spirulina and Ferrous Fumarate on Intestinal Morphology and the Diversity of Gut Microbiota of Yellow River Carp

The study aimed to investigate the effect of diets supplemented with Spirulina and the mixture of Spirulina and ferrous fumarate on intestinal morphology and the diversity of gut microbiota of Yellow River carp. The results showed that the Spirulina and the mixture of Spirulina and ferrous fumarate could promote the development of intestinal villi, increase the thickness of intestinal muscular layer. Additionally, high-throughput Sequencing of 16S rRNA gene revealed that the Spirulina and the mixture of Spirulina and ferrous fumarate could alter the composition, diversity, and richness of intestinal microbial communities. The relative abundances of the predominant phyla Firmicutes and Verrucomicrobia showed significant changes at the phylum level after fed with Spirulina and ferrous fumarate. At the genus level, the predominant genera with marked differences in abundances were Flavobacterium, Aeromonas, and Brevinema. In conclusion, this study indicated the Spirulina and the ferrous fumarate could alter the intestinal microbiota structure and could also cause positive impacts on the health of Yellow River carp. This study provides the valuable information for elucidating the mechanisms of Spirulina and ferrous fumarate in aquatic Animals in the future.

Ferrous fumarate; Gut microbiota; Intestinal morphology; Spirulina; Yellow River carp.