2. Academic Validation
  3. The modulating effect of methoxy-derivatives of 2'-hydroxychalcones on the release of IL-8, MIF, VCAM-1 and ICAM-1 by colon cancer cells

The modulating effect of methoxy-derivatives of 2'-hydroxychalcones on the release of IL-8, MIF, VCAM-1 and ICAM-1 by colon cancer cells

  • Biomed Pharmacother. 2022 Jan:145:112428. doi: 10.1016/j.biopha.2021.112428.
Joanna Bronikowska 1,  Małgorzata Kłósek 2,  Tomasz Janeczko 3,  Edyta Kostrzewa-Susłow 4,  Zenon P Czuba 5
Affiliations

Affiliations

  • 1 Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland. Electronic address: jbronikowska@sum.edu.pl.
  • 2 Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland. Electronic address: mklosek@sum.edu.pl.
  • 3 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland. Electronic address: tomasz.janeczko@upwr.edu.pl.
  • 4 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland. Electronic address: edyta.kostrzewa-suslow@upwr.edu.pl.
  • 5 Department of Microbiology and Immunology, Faculty of Medical Sciences, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland. Electronic address: zczuba@sum.edu.pl.
PMID: 34800781 DOI: 10.1016/j.biopha.2021.112428
Abstract

Colon Cancer is one of the leading causes of death in the world. The search for effective and minimally invasive methods of treating colon Cancer is the aim of modern medicine. Chalcones and their derivatives have shown an Anticancer activity. The aim of the study was to evaluate the effect of methoxy-derivatives of 2'-hydroxychalcones: 2'-hydroxy-3"-methoxychalcone (TJ3), 2'-hydroxy-2"-methoxychalcone (TJ6) and 2'-hydroxy-4"-metoxychalcone (TJ7) at the concentrations of 10 µM and 25 µM on the release of IL-8, MIF, VCAM-1, ICAM-1 by colon Cancer SW480 and SW620 cell lines. The cytokines and adhesion molecules were detected using the Bio-Plex Magnetic Luminex Assay and the Bio-Plex Suspension Array System. Our results showed that all tested methoxy-derivatives of 2'-hydroxychalcone compounds significantly reduced ICAM-1 released by SW480 Cancer cells. The tested compounds at both concentrations did not significantly affect VCAM-1 released by SW480 and SW620 Cancer cell lines. All methoxy-derivatives significantly reduced the concentration of MIF in dose dependent manner on SW480 cells. The TJ3 at the concentration of 25 µM significantly decreased IL-8 secreted by SW480 and SW620 Cancer cells. Our results demonstrated that tested methoxy-derivatives of 2'-hydroxychalcones showed modulating effect on colon Cancer cells.

Keywords

Adhesion molecules; Chalcone derivatives; Chemoprevention; Colon cancer; Cytokines.

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