Structural characterisation and antitumor activity against non-small cell lung cancer of polysaccharides from Sanghuangporus vaninii

Carbohydr Polym. 2022 Jan 15;276:118798. doi: 10.1016/j.carbpol.2021.118798.

Xilin Wan ¹, Xin Jin ², Xinmin Wu ³, Xue Yang ⁴, Dongmei Lin ⁵, Changtian Li ⁶, Yongping Fu ⁶, Yang Liu ⁶, Xinze Liu ⁷, Jianhua Lv ⁶, Andrey A Gontcharov ⁸, Hongmei Yang ⁷, Qi Wang ⁹, Yu Li ¹⁰

Affiliations

1 International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, China; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
2 Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, China.
3 Department of Neurosurgery, First Hospital of Jilin University, Changchun 130021, China.
4 Cardiovascular Medicine and Cardiac Rehabilitation Center, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021, China.
5 Electric Diagnosis Department, Jilin Municipal Hospital of Traditional Chinese Medicine, Jilin 132000, China.
6 International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, China.
7 Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
8 Institute of Biology and Soil Science, FEB RAS, 100-letia Vladivostoka Prospect, 159, Vladivostok 690022, Russia.
9 International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, China. Electronic address: qiwang@jlau.edu.cn.
10 International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun 130118, China. Electronic address: yuli966@126.com.

PMID: 34823804 DOI: 10.1016/j.carbpol.2021.118798

Abstract

The medicinal fungus Sanghuangporus vaninii can be cultivated in large scale and has outstanding antitumour activity. In this study, water-soluble S. vaninii Polysaccharides (SVPs) were extracted from fruiting bodies. Four polysaccharide sub-fractions (SVP-W, SVP-1, SVP-2 and SVP-3) were isolated, with molecular weights from 90.50 kDa to 261.70 kDa, and all inhibited the proliferation of non-small cell lung Cancer cell lines A549, 95-D and NCI-H460, especially the acidic SVP-1. SVP-1 affected cell morphology and colony formation in NCI-H460 cells. It also promoted cell Apoptosis following nuclear fluorescence staining and flow cytometry. Methylation and nuclear magnetic resonance analyses revealed that SVP-1 is a heteroglycan with the main chain →4)-β-D-Glcp-(1 → 6)-β-D-Glcp-(1 → 6)-α-D-Galp-(1 → 6)-β-D-Glcp-(1→, and the branched chain α-D-Manp-(1 → 2)-α-D-Manp-(1 → 3)-β-D-Glcp-(1 → 3,6)-β-D-Glcp-(1→. The findings indicate that this natural acidic polysaccharide has potential for non-small cell lung Cancer therapy.

Keywords

Antitumour activity; Lung cancer; Polysaccharide; Sanghuangporus vaninii; Structure.

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Cell Counting Kit-8

Cell Proliferation and Cytotoxicity

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