2. Academic Validation
  3. Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway

Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway

  • Acta Pharm Sin B. 2021 Nov;11(11):3465-3480. doi: 10.1016/j.apsb.2021.05.027.
Xiangjin Zheng 1 2,  Wan Li 1 2,  Huanli Xu 3,  Jinyi Liu 1 2,  Liwen Ren 1 2,  Yihui Yang 1 2,  Sha Li 1 2,  Jinhua Wang 1 2,  Tengfei Ji 2,  Guanhua Du 1 2
Affiliations

Affiliations

  • 1 The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China.
  • 2 Key Laboratory of Drug Target Research and Drug Screen, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China.
  • 3 Department of Pharmacology, Capital Medical University, Beijing 100069, China.
PMID: 34900530 DOI: 10.1016/j.apsb.2021.05.027
Abstract

Glioblastoma multiforme (GBM) in the central nervous system is the most lethal advanced glioma and currently there is no effective treatment for it. Studies of sinomenine, an alkaloid from the Chinese medicinal plant, Sinomenium acutum, showed that it had inhibitory effects on several kinds of Cancer. Here, we synthesized a sinomenine derivative, sino-wcj-33 (SW33), tested it for antitumor activity on GBM and explored the underlying mechanism. SW33 significantly inhibited proliferation and colony formation of GBM and reduced migration and invasion of U87 and U251 cells. It also arrested the cell cycle at G2/M phase and induced mitochondria-dependent Apoptosis. Differential gene enrichment analysis and pathway validation showed that SW33 exerted anti-GBM effects by regulating PI3K/Akt and AMPK signaling pathways and significantly suppressed tumorigenicity with no obvious adverse effects on the body. SW33 also induced Autophagy through the PI3K/Akt/mTOR and AMPK/mTOR pathways. Thus, SW33 appears to be a promising drug for treating GBM effectively and safely.

Keywords

Anti-inflammation; Apoptosis; Autophagy; G2/M phase; GBM; SW33; Safety; mTOR.

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