2. Academic Validation
  3. Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

  • J Periodontal Res. 2022 Apr;57(2):425-433. doi: 10.1111/jre.12973.
Xiaochi Chang 1 2 3,  Fengyi Zhou 1 2,  Lingxue Bu 4,  Nan Wang 2 5,  Jing Deng 1 2 3,  Shuai Wang 1 2 3
Affiliations

Affiliations

  • 1 Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, China.
  • 2 School of Stomatology of Qingdao University, Qingdao, China.
  • 3 Dental Digital Medicine & 3D Printing Engineering Laboratory of Qingdao, Qingdao, China.
  • 4 Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
  • 5 Department of Stomatology, Hospital of the PLA Navy, Qingdao, China.
PMID: 35037251 DOI: 10.1111/jre.12973
Abstract

Objective and background: The occurrence and development of periodontitis are closely related to hypoxia of the periodontal microenvironment. Periodontal ligament stem cells (PDLSCs) are considered to have potential to regenerate periodontal tissues. Semaphorin 3A (Sema3A) plays an essential role in promoting osteogenesis. However, the effect of Sema3A on osteogenesis of PDLSCs under hypoxia remains unclear. The aim of this study was to investigate the effect of Sema3A on osteogenesis of PDLSCs under hypoxia.

Methods: Isolated PDLSCs were identified using flow cytometry. Adipogenic differentiation potential was identified by oil red O staining. Osteogenesis was measured using Alizarin Red S staining and ALP staining. Intracellular hypoxia was induced using cobalt chloride (CoCl2 ). The expression level of hypoxia-inducible factor-1α (HIF-1α) was detected via ELISA. Expression of osteogenic markers and Sema3A was analyzed using western blot and Real-Time PCR.

Results: The proliferation and osteogenesis of PDLSCs were markedly inhibited with increased concentrations of CoCl2 . Under the treatment with a low concentration of CoCl2 , expression of related osteogenic markers and Sema3A decreased in a time-dependent manner. ARS and ALP staining results also showed that osteogenic calcification decreased under hypoxia. Apigenin, an inhibitor of HIF-1α, effectively up-regulated expression of Sema3A and osteogenic markers with CoCl2 treatment. Moreover, exogenous Sema3A significantly increased the expression of osteogenesis-related markers and mineralization of PDLSCs according to ALP and ARS staining with CoCl2 treatment.

Conclusions: Hypoxia markedly inhibited osteogenesis of PDLSCs. Sema3A explicitly attenuated the hypoxia suppression of osteogenesis in PDLSCs.

Keywords

hypoxia; osteogenesis; periodontal ligament stem cells; semaphorin 3A.

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