2. Academic Validation
  3. Long noncoding RNA GAS5 inhibits proliferation and metastasis in papillary thyroid carcinoma through the IFN/STAT1 signaling pathway

Long noncoding RNA GAS5 inhibits proliferation and metastasis in papillary thyroid carcinoma through the IFN/STAT1 signaling pathway

  • Pathol Res Pract. 2022 May;233:153856. doi: 10.1016/j.prp.2022.153856.
Yongcan Xu 1,  Jun Lu 2,  Neng Lou 3,  Wei Lu 4,  Jiewei Xu 5,  Hao Jiang 6,  Guochao Ye 7
Affiliations

Affiliations

  • 1 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: yongcanx@163.com.
  • 2 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: easonlo87@icloud.com.
  • 3 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: louneng0923@163.com.
  • 4 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: 13587291619@163.com.
  • 5 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: xujieweikof@126.com.
  • 6 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: jhao_1988@sina.com.
  • 7 Department of General Surgery, Huzhou Central Hospital, Affiliated Central Hospital, Huzhou University, Huzhou, China. Electronic address: chaogechina@163.com.
PMID: 35366462 DOI: 10.1016/j.prp.2022.153856
Abstract

Dysregulation of the long noncoding RNA GAS5 in human Cancer has been identified in recent studies. In this study, we confirmed a negative correlation between the GAS5 expression level and papillary thyroid carcinoma clinicopathologic characteristics, such as the tumor size, lymph node metastasis, the TNM stage and BRafV600E mutation. The viability and metastasis of papillary thyroid carcinoma cells were detected by CCK-8 and transwell assays, respectively. The results showed that upregulation of GAS5 significantly inhibited papillary thyroid Cancer cell growth, migration and invasion in vitro. RNA transcriptome Sequencing was performed to explore the underlying targets of GAS5. Through qRT-PCR and Western blot analysis, we found that ectopic expression of GAS5 significantly increased IFI44 and STAT1 levels. Taken together, these findings suggest that GAS5 is a tumor suppressor in papillary thyroid carcinoma, and the action of GAS5 may be mediated through the IFNγ/STAT1 signaling pathway.

Keywords

GAS5; IFI44; LncRNA; Papillary thyroid carcinoma.

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