2. Academic Validation
  3. Six C21 steroidal glycosides from Cynanchum wallichii Wight roots and their multidrug resistance reversal activities

Six C21 steroidal glycosides from Cynanchum wallichii Wight roots and their multidrug resistance reversal activities

  • Phytochemistry. 2022 Jul;199:113172. doi: 10.1016/j.phytochem.2022.113172.
Ludi Zhang 1,  Lin Yuefang 1,  Huang Min 1,  Chen Wenbo 1,  Lixin Duan 1,  Zhongqiu Liu 2,  Linlin Lu 3,  Rong-Rong Zhang 4
Affiliations

Affiliations

  • 1 Joint Laboratory for Translational Cancer Research on Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China.
  • 2 Joint Laboratory for Translational Cancer Research on Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China. Electronic address: liuzq@gzucm.edu.cn.
  • 3 Joint Laboratory for Translational Cancer Research on Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China. Electronic address: lllu@gzucm.edu.cn.
  • 4 Joint Laboratory for Translational Cancer Research on Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China. Electronic address: zrr586@gzucm.edu.cn.
PMID: 35381277 DOI: 10.1016/j.phytochem.2022.113172
Abstract

Six unidentified C21 steroidal glycosides, cynwallosides A-F, as well as twenty-two known compounds, were isolated from the roots of Cynanchum wallichii Wight. The structures of cynwallosides A-F were determined by spectroscopic analysis and acidic hydrolysis. Most of these twenty-eight compounds were found to significantly reverse drug resistance in both the MCF-7/ADR and HepG2/ADM cell lines by suppressing P-gp protein expression. Further investigation revealed that three compounds suppressed P-gp expression by significantly inactivating the JNK and NF-κB pathways.

Keywords

Asclepiadaceae; C(21) steroidal glycosides; Cynanchum wallichii Wight; Drug resistance; P-gp.

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