2. Academic Validation
  3. A plant-specific module for homologous recombination repair

A plant-specific module for homologous recombination repair

  • Proc Natl Acad Sci U S A. 2022 Apr 19;119(16):e2202970119. doi: 10.1073/pnas.2202970119.
Xuanpeng Wang 1 2,  Lili Wang 1 2,  Yongchi Huang 1 2,  Zhiping Deng 3,  Cunliang Li 1 2,  Jian Zhang 1 2,  Mingxi Zheng 1 2,  Shunping Yan 1 2
Affiliations

Affiliations

  • 1 Hubei Hongshan Laboratory, Wuhan, 430070, China.
  • 2 College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • 3 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
PMID: 35412914 DOI: 10.1073/pnas.2202970119
Abstract

Homologous recombination repair (HR) is an error-free DNA damage repair pathway to maintain genome stability and a basis of gene targeting using genome-editing tools. However, the mechanisms of HR in Plants are still poorly understood. Through genetic screens for DNA damage response mutants (DDRM) in Arabidopsis, we find that a plant-specific ubiquitin E3 Ligase DDRM1 is required for HR. DDRM1 contains an N-terminal BRCT (BRCA1 C-terminal) domain and a C-terminal RING (really interesting new gene) domain and is highly conserved in Plants including mosses. The ddrm1 mutant is defective in HR and thus is hypersensitive to DNA-damaging reagents. Biochemical studies reveal that DDRM1 interacts with and ubiquitinates the transcription factor SOG1, a plant-specific master regulator of DNA damage responses. Interestingly, DDRM1-mediated ubiquitination promotes the stability of SOG1. Consistently, genetic data support that SOG1 functions downstream of DDRM1. Our study reveals that DDRM1-SOG1 is a plant-specific module for HR and highlights the importance of ubiquitination in HR.

Keywords

DNA damage response; SOG1; homologous recombination repair; ubiquitination.

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