2. Academic Validation
  3. Choline Protects the Heart from Doxorubicin-Induced Cardiotoxicity through Vagal Activation and Nrf2/HO-1 Pathway

Choline Protects the Heart from Doxorubicin-Induced Cardiotoxicity through Vagal Activation and Nrf2/HO-1 Pathway

  • Oxid Med Cell Longev. 2022 Apr 5;2022:4740931. doi: 10.1155/2022/4740931.
Fuding Guo  # 1 2 3 4,  Yueyi Wang  # 1 2 3 4,  Jun Wang  # 1 2 3 4,  Zhihao Liu 1 2 3 4,  Yanqiu Lai 1 2 3 4,  Zhen Zhou 1 2 3 4,  Zihan Liu 1 2 3 4,  Yuyang Zhou 1 2 3 4,  Xiao Xu 1 2 3 4,  Zeyan Li 1 2 3 4,  Meng Wang 1 2 3 4,  Fu Yu 1 2 3 4,  Ruijie Hu 1 2 3 4,  Liping Zhou 1 2 3 4,  Hong Jiang 1 2 3 4
Affiliations

Affiliations

  • 1 Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.
  • 2 Cardiac Autonomic Nervous System Research Centre of Wuhan University, Wuhan, China.
  • 3 Cardiovascular Research Institute, Wuhan University, Wuhan, China.
  • 4 Hubei Key Laboratory of Cardiology, Wuhan, China.
  • # Contributed equally.
PMID: 35422894 DOI: 10.1155/2022/4740931
Abstract

Choline is a precursor of the major neurotransmitter acetylcholine and has been demonstrated beneficial in diverse models of Cardiovascular Disease. Here, we sought to verify that choline protects the heart from DOX-induced cardiotoxicity and the underlying mechanisms. The results showed that DOX treatment decreased left ventricular ejection fraction and fractional shortening and increased serum cardiac markers and myocardial fibrosis, which were alleviated by cotreatment with choline. DOX-induced cardiotoxicity was accompanied by increases in oxidative stress, inflammation, and Apoptosis, which were rectified by choline cotreatment. Levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme-oxygenase-1 (HO-1), which are antioxidant markers, were lowered by DOX and upregulated by choline. Moreover, DOX significantly decreased serum acetylcholine levels and the high-frequency component of heart rate variability and increased serum norepinephrine levels and the low-frequency component; these effects were rescued by choline administration. Interestingly, the protective effects of choline could be partially reversed by administration of the muscarinic receptor antagonist atropine. This suggests that choline might be a promising adjunct therapeutic agent to alleviate DOX-induced cardiotoxicity.

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