2. Academic Validation
  3. Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo

Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo

  • Food Chem X. 2022 Apr 30;14:100321. doi: 10.1016/j.fochx.2022.100321.
Xiong Gao 1,  Ranhua Zeng 2,  Jiayi Qi 2,  Chi-Tang Ho 3,  Bin Li 2 4,  Zhongzheng Chen 2 4,  Shaodan Chen 1,  Chun Xiao 1,  Huiping Hu 1,  Manjun Cai 1,  Yizhen Xie 1 5 6,  Qingping Wu 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
  • 2 College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, China.
  • 3 Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
  • 4 Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, South China Agricultural University, Guangzhou 510642, China.
  • 5 Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou 510663, China.
  • 6 Guangdong Yuewei Biotechnology Co. Ltd., Zhaoqing 526000, China.
PMID: 35571333 DOI: 10.1016/j.fochx.2022.100321
Abstract

The chemical structure of GLP-1, a novel water-soluble heteropolysaccharide purified Ganoderma leucocontextum fruiting bodies, has been characterized in our previous study. This study aimed to investigate the immunostimulatory activity of GLP-1 in vitro and in vivo by using RAW264.7 macrophages and cyclophosphamide-induced immunosuppressed mice model. Results showed that GLP-1 was able to enhance phagocytic activity and promote the production of Reactive Oxygen Species, nitric oxide, tumor necrosis factor-α, interleukin-6, and monocyte chemoattractant protein-1 in RAW264.7 macrophages. Moreover, GLP-1 could activate mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways through Toll-like Receptor 2 and Dectin-1 receptors. Furthermore, GLP-1 increased the thymus index, serum immunoglobulin levels, and percentage of CD3+ T lymphocytes in cyclophosphamide-induced immunosuppressed mice. These results demonstrated that GLP-1 possessed significant immunostimulatory effects in vivo and in vitro and could be developed as an effective immunomodulator for application in functional foods.

Keywords

Ganoderma leucocontextum; Heteropolysaccharide; Immunostimulatory activity; Immunosuppressed mice; Molecular mechanism.

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