2. Academic Validation
  3. Fluid shear stress induces cell migration via RhoA-YAP1-autophagy pathway in liver cancer stem cells

Fluid shear stress induces cell migration via RhoA-YAP1-autophagy pathway in liver cancer stem cells

  • Cell Adh Migr. 2022 Dec;16(1):94-106. doi: 10.1080/19336918.2022.2103925.
Zhiping Yan 1 2 3 4,  Danfeng Guo 1 2 3 4,  Ruolin Tao 1 2 3 4,  Xiao Yu 1 2 3 4,  Jiacheng Zhang 1 2 3 4,  Yuting He 1 2 3 4,  Jiakai Zhang 1 2 3 4,  Jie Li 1 2 3 4,  Shuijun Zhang 1 2 3 4,  Wenzhi Guo 1 2 3 4
Affiliations

Affiliations

  • 1 Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • 2 Henan Liver Transplantation Center, Zhengzhou, Henan Province, 450052, China.
  • 3 Henan Research Center for Organ Transplantation, Zhengzhou, 450052, China.
  • 4 Henan Key Laboratory of Digestive Organ Transplantation, Zhengzhou, 450052, China.
PMID: 35880618 DOI: 10.1080/19336918.2022.2103925
Abstract

Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC), but the role of the RhoA-YAP1-autophagy pathway in HCC remains unclear. Due to the core role of liver Cancer Stem Cells (LCSCs) in HCC metastasis and recurrence, we explored the RhoA-YAP1-autophagy pathway in LCSCs under FSS. Our results indicate that LCSCs have stronger proliferation and cell spheroidization abilities. FSS (1 dyn/cm2) upregulated the migration of LCSCs and Autophagy protein markers, inducing LC3B aggregation and autophagosome formation in LCSCs. Mechanistically, FSS promoted YAP1 dephosphorylation and transport to the nucleus, which is mediated by RhoA, inducing Autophagy. Finally, inhibition of Autophagy suppressed cell migration in LCSCs under FSS. In conclusion, FSS promoted the migration of LCSCs via the RhoA-YAP1-autophagy pathway.

Keywords

Fluid shear stress; LCSCs; autophagy; migration.

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