2. Academic Validation
  3. Establishment and characterization of a new Chinese hepatocellular carcinoma cell line, Hep-X1

Establishment and characterization of a new Chinese hepatocellular carcinoma cell line, Hep-X1

  • Hum Cell. 2022 Sep 24. doi: 10.1007/s13577-022-00797-z.
Hao Xu  # 1,  Xin Miao 2,  Changpeng Chai 3,  Huan Tang 3,  Jinjing Hu 3,  Zhenjie Zhao 3,  Wei Luo 3,  Kexiang Zhu 3,  Wence Zhou  # 4 5
Affiliations

Affiliations

  • 1 The Forth Department of General Surgery, The First Hospital of Lanzhou University, No.1 Donggang West Road, Chengguan District, Lanzhou, 730000, Gansu, China. amoyxu@126.com.
  • 2 State Key Laboratory of Veterinary Etiological Biology & OIE/National Foot and Mouth Disease Reference Laboratory & Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, LanZhou, 730000, China.
  • 3 The Forth Department of General Surgery, The First Hospital of Lanzhou University, No.1 Donggang West Road, Chengguan District, Lanzhou, 730000, Gansu, China.
  • 4 Department of General Surgery, The Second Hospital of Lanzhou University, Lanzhou, 730000, China. zhouwc129@163.com.
  • 5 The Second Clinical Medical College, Lanzhou University, Lanzhou, 730000, China. zhouwc129@163.com.
  • # Contributed equally.
PMID: 36152230 DOI: 10.1007/s13577-022-00797-z
Abstract

Hepatocellular carcinoma (HCC) is a highly aggressive and heterogeneous disease. Cell lines are commonly employed as in vitro models for cell type studies. However, the success rate of HCC primary culture establishment is low. In this study, we successfully established a liver Cancer cell line, Hep-X1. Primary culture and passage of surgically removed tissues were used to establish hepatoma cell lines. Morphological examination, short tandem repeat (STR) analysis, immunohistochemical staining, doubling time, karyotype analysis, plate tumor formation experiments, Organoid culture, and in vivo tumor formation investigations in Animals were used to identify the cell lines. A novel liver Cancer cell line, Hep-X1, was established based on morphology, immunophenotype, cytogenetics, and STR analysis. The novel cell line can be a valuable model for studying primary liver Cancer.

Keywords

Cell line; Liver cancer; Passage; Primary culture; Short tandem repeat.

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