Triton X-100

Name: Triton X-100
Brand: MedChemExpress
SKU: HY-Y1883A
Rating: 5.0 (10 reviews)

Cat. No.: HY-Y1883A Assay: 99.8%: FAQs
Data Sheet SDS COA Handling Instructions

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Triton X-100 is a non-denaturing detergent that solubilizes lipid membranes. Triton X-100 is commonly used in laboratories and is applied to vaccines at different stages of the manufacturing process. Triton X-100 is listed as an excipient in certain vaccines including split virus influenza vaccines. Triton X-100 is a nonionic surfactant.

For research use only. We do not sell to patients.

Triton X-100 Chemical Structure

CAS No. : 9002-93-1

Size	Price	Stock	Quantity
100 g	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 10 publication(s) in Google Scholar

Other Forms of Triton X-100:

Triton X-100 surfactant Get quote

10 Publications Citing Use of MCE Triton X-100

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Description

In Vitro

Triton X-100 (0.5%-0.05%, 1 h) reduces the infectivity of a classical enveloped virus such as West Nile virus (WNV)^[3].
Triton X-100 (0.19-0.20 mM) makes the HeLa cells underwent irreversible permeabilization of the membrane and structural collapse^[4].
Triton X-100 (0.01%, 1 h) induces apoptotic and necrotic death in prostate and colon cancer cell lines (PC-3, SW-620 and HT-29 cells), characterized by the typical morphological features and internucleosomal DNA fragmentation^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.

9002-93-1

Appearance

Liquid (Density: 1.065 g/cm³)

Color

Colorless to light yellow

SMILES

CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1.[n]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 200 mg/mL

DMSO : 100 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.8%

Select Batch:

Data Sheet (269 KB) SDS (758 KB)

COA (242 KB)

Handling Instructions (2659 KB)

References

[1]. Herman SM, et al. The hidden allergen: Triton X-100, a derivative of polyethylene glycol. J Allergy Clin Immunol Pract. 2021;9(7):2941. [Content Brief]

[2]. Ghosh R, et al. Unraveling diverse action of triton X-100 and methimazole on lysozyme fibrillation/aggregation: Physicochemical insights. Int J Biol Macromol. 2021;167:736-745. [Content Brief]

[3]. Colavita F, et al. Evaluation of the inactivation effect of Triton X-100 on Ebola virus infectivity. J Clin Virol. 2017 Jan;86:27-30. [Content Brief]

[4]. Koley D, et al. Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM). Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16783-7. [Content Brief]

[5]. Borner MM, et alE. The detergent Triton X-100 induces a death pattern in human carcinoma cell lines that resembles cytotoxic lymphocyte-induced apoptosis. FEBS Lett. 1994 Oct 17;353(2):129-32. [Content Brief]

Triton X-100 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triton X-1009002-93-1Triton X100Triton X 100Biochemical Assay Reagentsdetergentnonionic surfactantvaccinesmanufacturing processInhibitorinhibitorinhibit

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Triton X-100

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