2. Academic Validation
  3. ElNFS1, a nitroreductase gene from Enterobacter ludwigii, confers enhanced detoxification and phytoremediation of 4-nitrobenzaldehyde in rice

ElNFS1, a nitroreductase gene from Enterobacter ludwigii, confers enhanced detoxification and phytoremediation of 4-nitrobenzaldehyde in rice

  • Environ Pollut. 2022 Dec 1:314:120292. doi: 10.1016/j.envpol.2022.120292.
Zhenjun Li 1,  Jianjie Gao 1,  Yongsheng Tian 1,  Bo Wang 1,  Jing Xu 1,  Xiaoyan Fu 1,  Hongjuan Han 1,  Lijuan Wang 1,  Wenhui Zhang 1,  Yu Wang 1,  Yongdong Deng 1,  Zehao Gong 1,  Rihe Peng 1,  Quanhong Yao 2
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, 201106, PR China.
  • 2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, 201106, PR China. Electronic address: qhyao_saas@163.com.
PMID: 36181935 DOI: 10.1016/j.envpol.2022.120292
Abstract

4-nitrobenzaldehyde (4-NBA) is a widely used chemical intermediate for industrial application and an important photodegradation product of chloramphenicol. This compound represents a substantial threat to human health and ecosystem due to its genotoxic and mutagenic effect. In this study, the 4-NBA detoxification by transgenic rice overexpressing a Bacterial nitroreductase gene, ElNFS1, from Enterobacter ludwigii were investigated. The cytosol-targeted ElNFS1 transgenic Plants were selected to comprehensively examine their physio-biochemical responses and phytoremediation potential to 4-NBA. Our results showed that the transgenic Plants exhibited strong tolerance to 4-NBA. Overexpression of ElNFS1 could significantly alleviate 4-NBA-induced damages of photosynthetic apparatus and Reactive Oxygen Species overproduction in transgenic Plants. The phytoremediation assay revealed that transgenic Plants could remove more 4-NBA from the medium than wild-type Plants. HPLC and LC-MS assays showed that 4-aminobenzaldehyde was found in the reductive products of 4-NBA. Altogether, the function of ElNFS1 during 4-NBA detoxification was characterized for the first time, which provides a strong theoretical support for the application potential of ElNFS1 transgenic Plants on the phytoremediation of 4-NBA.

Keywords

4-Nitrobenzaldehyde; Nitroreductase; Photosynthetic apparatus; Phytoremediation; ROS.

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