1. Academic Validation
  2. Electrochemical sensor for detecting streptomycin in milk based on label-free aptamer chain and magnetic adsorption

Electrochemical sensor for detecting streptomycin in milk based on label-free aptamer chain and magnetic adsorption

  • Food Chem. 2022 Sep 26;403:134399. doi: 10.1016/j.foodchem.2022.134399.
Li Wang 1 Hongsu Wang 1 Shuqing Huang 1 Fengling Wu 1 Xiaodi Niu 2
Affiliations

Affiliations

  • 1 College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
  • 2 College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: niuxd@jlu.edu.cn.
Abstract

Exploiting a simple and sensitive sensor to efficiently detect streptomycin (STR) in milk is critical for resolving the harm caused to humans by STR residues. This study reports an electrochemical sensor using magnetic mesoporous carbon Materials (MMCM) as a loaded material through magnetic adsorption immobilized on magnetic glassy carbon electrode (MGCE) and adsorbing unlabeled streptomycin aptamer (STP) as the identification element. The sensor can detect STR sensitively with a wide detection range (0.172-17.2 × 103 and 17.2 × 103 -17.2 × 105 nM) and a low detection limit of 0.015 nM. Experimental results revealed that the specific binding of STP with STR on the electrode changed the configuration of STP, thereby causing current change of differential pulse voltammetry curve. Compared with HPLC, this study provides a new method for rapid and sensitive detection of STR in milk (n = 5, 95 % confidence level, RSD<5%).

Keywords

Differential pulse voltammetry; Electrochemical aptamer sensor; Label-free; Magnetic mesoporous carbon material; Streptomycin.

Figures
Products