2. Anti-infection
  3. Bacterial Antibiotic
  4. Tobramycin

Tobramycin  (Synonyms: Nebramycin Factor 6; Deoxykanamycin B)

Cat. No.: HY-B0441 Purity: 99.80%
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Tobramycin (Nebramycin Factor 6) is a parenterally administered, broad spectrum aminoglycoside antibiotic that is widely used in the treatment of moderate to severe bacterial infections due to sensitive organisms.Tobramycin can be used to pneumonia research caused by Pseudomonas aeruginosa.

For research use only. We do not sell to patients.

Tobramycin Chemical Structure

Tobramycin Chemical Structure

CAS No. : 32986-56-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 66 In-stock
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500 mg USD 120 In-stock
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Based on 7 publication(s) in Google Scholar

Other Forms of Tobramycin:

Tobramycin Related Antibodies

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Description

Tobramycin (Nebramycin Factor 6) is a parenterally administered, broad spectrum aminoglycoside antibiotic that is widely used in the treatment of moderate to severe bacterial infections due to sensitive organisms[1].Tobramycin can be used to pneumonia research caused by Pseudomonas aeruginosa[2][3].

IC50 & Target

Aminoglycoside

 

Cellular Effect
Cell Line Type Value Description References
Vero IC50
> 200 μg/mL
Compound: Tobramycin
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
[PMID: 28291943]
In Vitro

Tobramycin (0-50 ng/mL; 24 hours) combinates with mycobacterium fortuitum enzyme (PodA) can greatly decreases P. aeruginosa cell viability[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tobramycin Related Antibodies

Cell Viability Assay[2]

Cell Line: Pseudomonas aeruginosa
Concentration: 2,10,50 ng/mL
Incubation Time: 24 h
Result: Greatly decreased cell viability compared to no protein or inactive mycobacterium fortuitum enzyme (PodA) controls while PodA10 alone did not increase cell death.
In Vivo

Tobramycin (50-400 mg/kg/day, i.p., once every 4h) combinates with Meropenem (HY-13678) produces bacterial cell kill effect at low doses of both drugs in murine pneumonia model. [3].
Tobramycin (s.c., single dose) LD50 values in mice and rats are 441 and 969 mg/kg, respectively[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Murine Model of Pseudomonas aeruginosa Pneumonia Female, Swiss-Webster mice [3]
Dosage: 50, 100, 150, 214, and 400 mg/kg/day
Administration: Intraperitoneal injection (i.p.) ,once every 4h
Result: Had near-maximal killing of the wild-type bacteria occurred at approximately 150 mg/kg/day when tobramycin alone.
Combinated with Meropenem (HY-13678) produced near-maximal effect (i.e., bacterial cell kill) at low doses of both drugs (60 and 50 mg/kg/day for Meropenem (HY-13678) and tobramycin, respectively).
Animal Model: Mice, rats, cats and dogs for toxicologic evaluation[4]
Dosage: 7.5,15,30,120,441,969 mg/kg
Administration: Subcutaneous injection (s.c.), Intravenous injection (i.v.) ,Intramuscular injection(i.m.)
Result: The s.c. LD50 values in mice and rats were 441 and 969 mg/kg, respectively.
Within 1 hour after treated, death with central nervous system depression as a precursor occurred in rats and mice.
A 100 mg/kg iv dose in chloraloseanesthetized catsproduced a moderate, transient decreasein blood pressure and a significant decrease in inspiratory volume and soleus twitch force.
Changed renal tissue in rats which were given daily sc doses of 15-120 mg/kg for 3 months.
im dose of 7.5 mg/kg for a l-month had no apparent effect on dogs, but a 30 mg/kg dose for a l-month produced severerenal damage.
Clinical Trial
Molecular Weight

467.51

Formula

C18H37N5O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@H]1O[C@@]([C@@H](C[C@@H]2O)N)([H])O[C@@H]2CN)[C@H]([C@@H](C[C@@H]1N)N)O[C@@]([C@@H]([C@@H](N)[C@@H]3O)O)([H])O[C@@H]3CO
Structure Classification
Initial Source

Streptomyces tenebrarius
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (213.90 mM)

DMSO : 2 mg/mL (4.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1390 mL 10.6950 mL 21.3899 mL
5 mM 0.4278 mL 2.1390 mL 4.2780 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (213.90 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.80%

SDS (418 KB)

COA (252 KB) RP-HPLC (164 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.1390 mL 10.6950 mL 21.3899 mL 53.4748 mL
H2O 5 mM 0.4278 mL 2.1390 mL 4.2780 mL 10.6950 mL
10 mM 0.2139 mL 1.0695 mL 2.1390 mL 5.3475 mL
15 mM 0.1426 mL 0.7130 mL 1.4260 mL 3.5650 mL
20 mM 0.1069 mL 0.5347 mL 1.0695 mL 2.6737 mL
25 mM 0.0856 mL 0.4278 mL 0.8556 mL 2.1390 mL
30 mM 0.0713 mL 0.3565 mL 0.7130 mL 1.7825 mL
40 mM 0.0535 mL 0.2674 mL 0.5347 mL 1.3369 mL
50 mM 0.0428 mL 0.2139 mL 0.4278 mL 1.0695 mL
60 mM 0.0356 mL 0.1782 mL 0.3565 mL 0.8912 mL
80 mM 0.0267 mL 0.1337 mL 0.2674 mL 0.6684 mL
100 mM 0.0214 mL 0.1069 mL 0.2139 mL 0.5347 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tobramycin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tobramycin32986-56-4Nebramycin Factor 6 Deoxykanamycin BNebramycin Factor6Nebramycin Factor-6BacterialAntibioticPseudomonas aeruginosaPneumoniaCombined useInhibitorinhibitorinhibit

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