2. Academic Validation
  3. HELQ suppresses migration and proliferation of non-small cell lung cancer cells by repairing DNA damage and inducing necrosis

HELQ suppresses migration and proliferation of non-small cell lung cancer cells by repairing DNA damage and inducing necrosis

  • Cell Biol Int. 2022 Oct 2. doi: 10.1002/cbin.11922.
Nan Shan Zhong 1 2,  Wei Lai Tong 1 2,  Yu Zhang 1 2,  Shi Ning Xiao 1 2,  Jia Ming Liu 2,  An An Li 1 2,  Ge Liang Yao 1 2,  Qing Lin 3,  Zhi Li Liu 2
Affiliations

Affiliations

  • 1 Medical Innovation Center, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China.
  • 2 Institute of Spine and Spinal Cord, Nanchang University, Nanchang, P.R. China.
  • 3 Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Massachusetts, USA.
PMID: 36183369 DOI: 10.1002/cbin.11922
Abstract

HELQ plays a key role in DNA damage response and cell-cycle checkpoint regulation. It has been implicated in ovarian and pituitary tumors and may play a role in germ cell maintenance. This study investigated the role of HELQ in lung Cancer. The expression of HELQ in patients with non-small-cell lung Cancer (NSCLC) was downregulated compared with normal human lungs. Clinical prognostic analysis of Kaplan-Meier plots revealed that patients with NSCLC with low HELQ levels had a reduced overall survival. Further, we found that HELQ depletion enhanced lung Cancer cell malignancy. Furthermore, overexpression of HELQ in lung Cancer cells reduced cell migration in vitro, while DNA damage repair was inhibited. Both in vitro and in vivo studies have shown that HELQ induces cell death. Mechanistically, we found that cells overexpressing HELQ showed a tendency to induce necrosis. After analyzing the database of HELQ interactors. we found that RIPK3 may interact with it and proved this conclusion by immunoprecipitation. Our findings identified the tumor suppressive role of HELQ in malignant human lung Cancer and unraveled a potential therapeutic strategy for Cancer treatment through HELQ activation. Moreover, HELQ may also be a predictive biomarker for the clinical predisposition, progression, and prognosis of lung Cancer.

Keywords

DNA damage; biomarker; necrosis; non-small-cell lung cancer; prognosis.

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