1. Academic Validation
  2. Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

  • Drug Dev Res. 2022 Oct 2. doi: 10.1002/ddr.22000.
Yiyu Qin 1 Mingdi Zhang 2 Haiming Lei 1 Hongyan Wu 1 Cheng Huang 1 Xin Zhou 3 Yang Fu 4 Mingzhe Weng 5 Mingzhe Ma 6
Affiliations

Affiliations

  • 1 Clinical Medical College, Jiangsu Vocational College of Medicine, Yancheng, China.
  • 2 Department of Breast Surgery, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
  • 3 Department of General Surgery, Xiangtan Central Hospital, Xiangtan, China.
  • 4 Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • 5 Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • 6 Department of Gastric Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
Abstract

Gallbladder Cancer (GBC) ranks seventh among the gastrointestinal cancers. Messenger RNAs (mRNAs) could regulate the progression of GBC. For the purpose of exploring the targets for GBC treatment, RNA Sequencing was used to identify the differential expressed mRNAs between GBC and adjacent tissues. Next, CCK8 assay was used to assess the cell viability, and cell proliferation was investigated by colony formation assay. Flow cytometry was performed to evaluate the cell Apoptosis. Protein and mRNA expression were analyzed by western blot and RT-qPCR, respectively. Transwell was performed to evaluate the cell metastasis. GBC-derived exosomes were isolated with ultracentrifugation. To evaluate the function of exosomes in GBC, in vivo model of GBC was constructed. The data revealed IGF2BP3 was identified to be upregulated in GBC, and IGF2BP3 silencing was able to decrease GBC cell proliferation by promoting the Apoptosis. The migration and invasion of GBC cells were reduced by IGF2BP3 knockdown. Silencing of IGF2BP3 obviously suppressed the level of p-STAT3 in GBC cells. Meanwhile, GBC cell-derived exosomes notably promoted macrophage M2 polarization via carrying IGF2BP3, and then the polarized macrophages promoted the malignant behavior of GBC cells. Furthermore, exosomes markedly promoted the tumor growth of GBC via promoting macrophage M2 polarization. In summary, knockdown of IGF2BP3 suppressed the malignant behavior of GBC cells. Additionally, knockdown of IGF2BP3 modified tumor microenvironment during the progression of GBC. Thus, these findings might provide a new theoretical basis for exploring a strategies against GBC.

Keywords

GBC; IGF2BP3; STAT3; exosome; macrophages.

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