2. Academic Validation
  3. Excess KLHL24 impairs skin wound healing by degradation of vimentin

Excess KLHL24 impairs skin wound healing by degradation of vimentin

  • J Invest Dermatol. 2023 Jan 27;S0022-202X(23)00025-8. doi: 10.1016/j.jid.2023.01.007.
Yihe Liu 1,  Jun Cui 2,  Jing Zhang 2,  Zhiming Chen 1,  Zhongya Song 1,  Dan Bao 3,  Ruiyu Xiang 1,  Dongqing Li 1,  Yong Yang 4
Affiliations

Affiliations

  • 1 Genetic Skin Disease Center, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, China; Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, Jiangsu, China.
  • 2 Department of Dermatology, Peking University First Hospital, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses and National Clinical Research Center for Skin and Immune Diseases, Beijing, China.
  • 3 Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, China.
  • 4 Genetic Skin Disease Center, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, China; Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, Jiangsu, China. Electronic address: yyang@pumcderm.cams.cn.
PMID: 36716923 DOI: 10.1016/j.jid.2023.01.007
Abstract

Start codon mutations in ubiquitin Ligase Kelch-like protein 24 (KLHL24) lead to a gain-of-function mutant KLHL24-ΔN28, which mediates the excessive degradation of keratin 15, desmin, and keratin 14, resulting in alopecia, cardiopathy, and epidermolysis bullosa syndrome (ACES). ACES patients normally present atrophic scars after wounds heal, which is rare in KRT14-related epidermolysis bullosa (EB). The mechanisms underlying the formation of atrophic scars in EB of ACES patients remain unclear. This study demonstrated that KLHL24-ΔN28 impaired skin wound healing by excessively degrading vimentin. Heterozygous Klhl24c.3G>T knock-in mice displayed delayed wound healing and decreased wound collagen deposition. We identified vimentin as a unreported substrate of KLHL24. KLHL24-ΔN28 mediated the excessive degradation of vimentin, which failed to maintain efficient fibroblast proliferation and activation during wound healing. Furthermore, by mediating vimentin degradation, KLHL24 can hinder myofibroblast activation, which attenuated bleomycin-induced skin fibrosis. These findings demonstrated the function of KLHL24 in regulating tissue remodeling, atrophic scarring, and fibrosis.

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