2. Academic Validation
  3. Structural characterization and immunomodulatory activity of an arabinogalactan from Jasminum sambac (L.) Aiton tea processing waste

Structural characterization and immunomodulatory activity of an arabinogalactan from Jasminum sambac (L.) Aiton tea processing waste

  • Int J Biol Macromol. 2023 Feb 23;123816. doi: 10.1016/j.ijbiomac.2023.123816.
Hai Huang 1,  Xiang Yang 1,  Wei Li 2,  Qifeng Han 1,  Zhizhen Xu 1,  Wei Xia 1,  Mengqi Wu 3,  Wenqing Zhang 4
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
  • 2 State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, PR China.
  • 3 Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: wumengqi@ecust.edu.cn.
  • 4 Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: zhwqing@ecust.edu.cn.
PMID: 36841385 DOI: 10.1016/j.ijbiomac.2023.123816
Abstract

An arabinogalactan named JSP-1a was isolated from Jasmine tea processing waste by DEAE Sepharose FF and Sephacryl S-200 HR chromatography. Polysaccharide JSP-1a, with an average molecular weight of 87.5 kDa, was composed of galactose (59.60 %), arabinose (33.89 %), mannose (4.81 %), and rhamnose (1.70 %). JSP-1a was found to be a type II arabinogalactan comprising the main backbone of 1, 6-linked Galp residues, and the side chain containing α-T-Araf, α-1,5-Araf, β-T-Galp, β-1,3-Galp, and β-1,4-Manp residues was attached to the O-3 position of β-1,3,6-Galp residues. Evidence from bioactivity assays indicated that JSP-1a possessed potent immunomodulatory effects on RAW264.7 macrophages: treatment with JSP-1a increased phagocytosis, activated NF-κB p65 translocation, and promoted the production of NO, Reactive Oxygen Species (ROS), the tumor necrosis factor (TNF)-α, and interleukin (IL)-6. Furthermore, inhibition of Toll-like Receptor 4 caused the suppression of NO release and cytokines secretion, which indicated that TLR-4/NF-κB pathway might play a significant role in JSP-1a-induced macrophages' immune response. The results of this study could provide a theoretical basis of JSP-1a as a safe immunostimulatory functional foods or a treatment for immunological diseases.

Keywords

Chemical structure; Immunomodulatory activity; Jasminum sambac (L.) Aiton tea; Polysaccharide; Processing waste; RAW264.7 macrophages.

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