2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. C29

C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively.

For research use only. We do not sell to patients.

C29 Chemical Structure

C29 Chemical Structure

CAS No. : 363600-92-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 188 In-stock
Solution
10 mM * 1 mL in DMSO USD 188 In-stock
Solid
1 mg USD 60 In-stock
5 mg USD 130 In-stock
10 mg USD 230 In-stock
50 mg USD 550 In-stock
100 mg USD 850 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 83 publication(s) in Google Scholar

C29 Related Antibodies

Top Publications Citing Use of Products

80 Publications Citing Use of MCE C29

WB
IF

    C29 purchased from MedChemExpress. Usage Cited in: J Control Release. 2020 Dec 10;328:210-221.  [Abstract]

    Western blot analysis show that MyD88 is active in DP7-C-stimulated BMDCs, and the activation of MyD88 expression by DP7-C and DOTAP/DP7-C is inhibited after BMDCs are pretreated with C29.

    C29 purchased from MedChemExpress. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Images show the expression of MCPIP1 after the treatment of of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    C29 purchased from MedChemExpress. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Western Blot analyses show the protein expression of MCPIP1 after the treatment of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    View All Toll-like Receptor (TLR) Isoform Specific Products:

    View All Isoforms
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    C29 is a Toll-like receptor 2 (TLR2) inhibitor. C29 blocks hTLR2/1 and hTLR2/6 signaling with IC50s of 19.7 and 37.6 μM, respectively[1].

    IC50 & Target[1]

    TLR2

     

    In Vitro

    C29 (10 or 50 μΜ; 1 hour) blocks P3C- and P2C-induced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 (50-200 μM; 1 hour) inhibits P3C- and P2C-induced IL-1β gene expression significantly at both 1 h and 4 h following stimulation in THP-1 cells[1].
    C29 (25 or 50 μΜ; 1 hour) reduces P3C-induced but not P2C-induced TNF-α mRNA and IL-12 p40 protein significantly in primary murine macrophages[1].
    C29 (50 μΜ; 1 hour) blocks TLR2 bacterial agonist-induced proinflammatory gene expression in HEK-TLR2 cells and murine macrophages[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    C29 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: THP-1 cells
    Concentration: 150 μM
    Incubation Time: 1 hours
    Result: Diminished the interaction between endogenous TLR2 and myeloid differentiation primary response gene 88 (MyD88) at 15 and 30 min poststimulation with P3C.

    Western Blot Analysis[1]

    Cell Line: Murine peritoneal macrophages
    Concentration: 50 μM
    Incubation Time: 1 hours
    Result: Blocked robust MAPK activation at 30 min and reduced NF-κB activation from 5 to 30 min.
    Prevented P3C-induced degradation of IκBα at 15 and 30 min.
    Molecular Weight

    285.29

    Formula

    C16H15NO4
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    OC1=C(/C=N/C2=C(C)C(C(O)=O)=CC=C2)C=CC=C1OC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (105.16 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5052 mL 17.5260 mL 35.0521 mL
    5 mM 0.7010 mL 3.5052 mL 7.0104 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5052 mL 17.5260 mL 35.0521 mL 87.6301 mL
    5 mM 0.7010 mL 3.5052 mL 7.0104 mL 17.5260 mL
    10 mM 0.3505 mL 1.7526 mL 3.5052 mL 8.7630 mL
    15 mM 0.2337 mL 1.1684 mL 2.3368 mL 5.8420 mL
    20 mM 0.1753 mL 0.8763 mL 1.7526 mL 4.3815 mL
    25 mM 0.1402 mL 0.7010 mL 1.4021 mL 3.5052 mL
    30 mM 0.1168 mL 0.5842 mL 1.1684 mL 2.9210 mL
    40 mM 0.0876 mL 0.4382 mL 0.8763 mL 2.1908 mL
    50 mM 0.0701 mL 0.3505 mL 0.7010 mL 1.7526 mL
    60 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4605 mL
    80 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0954 mL
    100 mM 0.0351 mL 0.1753 mL 0.3505 mL 0.8763 mL
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    C29 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    C29363600-92-4C 29C-29Toll-like Receptor (TLR)Toll-likeTLR2hTLR2/1hTLR2/6Inhibitorinhibitorinhibit

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