2. Academic Validation
  3. Sympathetic β2-adrenergic receptor blockade overcomes docetaxel resistance in prostate cancer

Sympathetic β2-adrenergic receptor blockade overcomes docetaxel resistance in prostate cancer

  • Biochem Biophys Res Commun. 2023 Mar 21;657:69-79. doi: 10.1016/j.bbrc.2023.03.046.
Mi Zhang 1,  Fangfang Chen 1,  Xueqing Sun 2,  Yanping Huang 1,  Yan Zeng 1,  Jinying Chen 1,  Shiqi Wu 1,  Chen Xu 3
Affiliations

Affiliations

  • 1 Institution of Life Science, Chongqing Medical University, Chongqing, China.
  • 2 Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • 3 Institution of Life Science, Chongqing Medical University, Chongqing, China. Electronic address: xuchen@cqmu.edu.cn.
PMID: 36989842 DOI: 10.1016/j.bbrc.2023.03.046
Abstract

Purpose: Due to the limited effective therapies, resistance to docetaxel is ordinarily fatal and remains a critical clinical challenge.β2-adrenergic receptor(β2-AR)can promote the metastasis and invasion of prostate Cancer, but the role in chemotherapy-resistant prostate Cancer remains unclear.

Methods: By downloading the GEO database in NCBI, the expression of β2-AR in different prostate tissues was analyzed. We constructed docetaxel-resistant prostate Cancer cell lines by the method of dose-escalation. LC3B-labeled stable cells and shAtg5 knockdown stable cells were constructed by lentivirus Infection. The molecular mechanism of β2-AR affecting docetaxel sensitivity through Apoptosis and autophage were investigated by flow cytometry, mitochondrial membrane potential and western blot. Then we detected the interaction between Autophagy and apoptotic by performing immunoprecipitation assay.

Results: We show that restraining the activity of β2-AR sensitized the cell response and reduced the resistance to docetaxel. The mechanism involves the regulation of β2-AR in the cellular response to docetaxel through Apoptosis and Autophagy via Caspase signaling and Atg5/AMPK/mTOR pathway as well as the effect of β2-AR on the crosstalk between Apoptosis and Autophagy via p38 MAPK and JNK/c-Jun/FOXO3a signaling pathways.

Conclusion: Our data demonstrate that β2-AR inhibitor-induced Autophagy and Apoptosis contribute to the effectiveness responses to docetaxel in castration-resistant prostate Cancer, and in combination with pharmacological agents of β2-AR and Autophagy inhibitors may provide a potential therapeutic strategy to enhance the limited capacity of docetaxel to control castration-resistant prostate Cancer.

Keywords

Apoptosis and autophagy; Chemotherapy resistance; Prostate cancer; β(2)-adrenergic receptor.

Figures
Products
Inhibitors & Agonists
Other Products